中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
Chinese Journal of Anesthesiology
2015年
7期
798-800
,共3页
敖吉莹%汤晓红%丁玲%李依泽%王志芬%王国林
敖吉瑩%湯曉紅%丁玲%李依澤%王誌芬%王國林
오길형%탕효홍%정령%리의택%왕지분%왕국림
麻醉药,吸入%婴儿,新生%cAMP反应元件结合蛋白质%海马%神经元
痳醉藥,吸入%嬰兒,新生%cAMP反應元件結閤蛋白質%海馬%神經元
마취약,흡입%영인,신생%cAMP반응원건결합단백질%해마%신경원
Anesthetics,inhalation%Infant,newborn%Cyclic AMP response element-binding protein%Hippocampus%Neurons
目的 评价七氟醚麻醉对发育期大鼠海马神经元磷酸化cAMP反应元件结合蛋白(p-CREB)表达的影响.方法 清洁级健康雄性SD大鼠32只,7日龄,体重10～ 15 g,采用随机数字表法分为2组(n=16):对照组(C组)吸入30％氧气6h;七氟醚麻醉组(Sev组)吸入30％氧气和3％七氟醚的混合气体6h.于吸氧或七氟醚麻醉结束后各组随机处死8只大鼠,取海马组织,采用Western blot法检测神经元p-CREB的表达.其余大鼠于2月龄时行Morris水迷宫实验,随后处死大鼠取海马组织,采用Western blot法检测神经元p-CREB的表达水平.结果 与C组比较,Sev组大鼠逃避潜伏期和游泳总路程延长,原平台穿越次数减少,第Ⅱ象限停留时间百分比降低,海马神经元p-CREB表达下调(P＜0.05).结论 七氟醚麻醉对发育期大鼠产生神经毒性作用的机制与其抑制海马神经元p-CREB表达有关.
目的 評價七氟醚痳醉對髮育期大鼠海馬神經元燐痠化cAMP反應元件結閤蛋白(p-CREB)錶達的影響.方法 清潔級健康雄性SD大鼠32隻,7日齡,體重10～ 15 g,採用隨機數字錶法分為2組(n=16):對照組(C組)吸入30％氧氣6h;七氟醚痳醉組(Sev組)吸入30％氧氣和3％七氟醚的混閤氣體6h.于吸氧或七氟醚痳醉結束後各組隨機處死8隻大鼠,取海馬組織,採用Western blot法檢測神經元p-CREB的錶達.其餘大鼠于2月齡時行Morris水迷宮實驗,隨後處死大鼠取海馬組織,採用Western blot法檢測神經元p-CREB的錶達水平.結果 與C組比較,Sev組大鼠逃避潛伏期和遊泳總路程延長,原平檯穿越次數減少,第Ⅱ象限停留時間百分比降低,海馬神經元p-CREB錶達下調(P＜0.05).結論 七氟醚痳醉對髮育期大鼠產生神經毒性作用的機製與其抑製海馬神經元p-CREB錶達有關.
목적 평개칠불미마취대발육기대서해마신경원린산화cAMP반응원건결합단백(p-CREB)표체적영향.방법 청길급건강웅성SD대서32지,7일령,체중10～ 15 g,채용수궤수자표법분위2조(n=16):대조조(C조)흡입30％양기6h;칠불미마취조(Sev조)흡입30％양기화3％칠불미적혼합기체6h.우흡양혹칠불미마취결속후각조수궤처사8지대서,취해마조직,채용Western blot법검측신경원p-CREB적표체.기여대서우2월령시행Morris수미궁실험,수후처사대서취해마조직,채용Western blot법검측신경원p-CREB적표체수평.결과 여C조비교,Sev조대서도피잠복기화유영총로정연장,원평태천월차수감소,제Ⅱ상한정류시간백분비강저,해마신경원p-CREB표체하조(P＜0.05).결론 칠불미마취대발육기대서산생신경독성작용적궤제여기억제해마신경원p-CREB표체유관.
Objective To evaluate the effect of sevoflurane anesthesia on the expression of phosphorylated cAMP response element-binding protein (p-CREB) in the hippocampal neurons of developing rats.Methods Thirty-two healthy male Sprague-Dawley rats, aged 7 days, weighing 10-15 g, were equally and randomly divided into either control group (group C) or sevoflurane anesthesia group (group Sev) using a random number table.Group C inhaled 30％ oxygen for 6 h.Group Sev inhaled 3％ sevoflurane for 6 h.Eight rats in each group were sacrificed immediately after the end of oxygen or sevoflurane inhalation, and the hippocampus was removed for determination of the expression of p-CREB.The rats at ages 2 months underwent Morris water maze test.The rats were then sacrificed, and the hippocampus was removed for determination of the expression of p-CREB by Western blot.Results Compared with group C, the escape latency and swimming distance were significantly prolonged, the frequency of crossing the original platform was decreased, the percentage of the time of staying at the quadrant Ⅱ was decreased, and the expression of p-CREB in hippocampal neurons was down-regulated in group Sev.Conclusion The mechanism of sevoflurane anesthesia-induced neurotoxicity is related to inhibition of p-CREB expression in hippocampal neurons of developing rats.
