CAJ | 학술논문

目的评价星状神经节阻滞(SGB)对脓毒症大鼠急性肺损伤的影响.方法健康雄性SD大鼠90只,体重300～ 350 g,12～15周龄,采用随机数字表法,将其分为3组(n=30):假手术组(S组)、脓毒症组(Sep组)和SGB组.采用颈交感神经干离断法行右侧SGB,30 min后采用盲肠结扎穿孔(CLP)法制备大鼠脓毒症模型.分别于CLP前5 min(T0)、CLP后2、6、12和24 h(T1-4)时每组取6只大鼠,采集腹主动脉血样,采用ELISA法检测血清TNF-α、IL-6和IL-10的浓度;于每次采集血样后,处死大鼠,取啼组织,测定湿/干重(W/D)比值,光镜下行肺组织病理学损伤评分,采用分光光度法测定髓过氧化物酶(MPO)活性,采用Westen blot法测定NF-κB p65表达.结果与S组比较,Sep组和SGB组T14时血清TNF-α、IL-6、IL-10浓度、肺组织W/D比值、病理学损伤评分和MPO活性升高,NF-κB p65表达上调(P＜0.05);与Sep组比较,SGB组T14时血清TNF-α 、IL-6浓度、肺组织W/D比值、病理学损伤评分和MPO活性降低,血清IL-10浓度升高,NF-κB p65表达下调(P＜0.05).结论 SGB可减轻脓毒症大鼠急性肺损伤.
목적 평개성상신경절조체(SGB)대농독증대서급성폐손상적영향.방법 건강웅성SD대서90지,체중300～ 350 g,12～15주령,채용수궤수자표법,장기분위3조(n=30):가수술조(S조)、농독증조(Sep조)화SGB조.채용경교감신경간리단법행우측SGB,30 min후채용맹장결찰천공(CLP)법제비대서농독증모형.분별우CLP전5 min(T0)、CLP후2、6、12화24 h(T1-4)시매조취6지대서,채집복주동맥혈양,채용ELISA법검측혈청TNF-α、IL-6화IL-10적농도;우매차채집혈양후,처사대서,취제조직,측정습/간중(W/D)비치,광경하행폐조직병이학손상평분,채용분광광도법측정수과양화물매(MPO)활성,채용Westen blot법측정NF-κB p65표체.결과 여S조비교,Sep조화SGB조T14시혈청TNF-α、IL-6、IL-10농도、폐조직W/D비치、병이학손상평분화MPO활성승고,NF-κB p65표체상조(P＜0.05);여Sep조비교,SGB조T14시혈청TNF-α 、IL-6농도、폐조직W/D비치、병이학손상평분화MPO활성강저,혈청IL-10농도승고,NF-κB p65표체하조(P＜0.05).결론 SGB가감경농독증대서급성폐손상.
Objective To evaluate the effect of stellate ganglion block (SGB) on acute lung injury in septic rats.Methods Ninety male Sprague-Dawley rats, aged 12-15 weeks, weighing 300-350 g, were equally and randomly divided into 3 groups using a random number table : sham operation group (group S) , sepsis group (group Sep) and SGB group.Cervical sympathetic trunk transaction was performed to induce right SGB in group SGB, and 30 min later sepsis was produced by cecum ligation and puncture (CLP) in CLP and SGB groups.At 5 min before CLP (T0) and 2, 6, 12 and 24 h after CLP (T1-4) , 6 rats were selected in each group, and blood samples were taken from the abdominal aorta for determination of the levels of tumor necrosis factor-alpha (TNF-α) , interleukin-6 (IL-6) and IL-10 in serum by enzyme-linked immunosorbent assay.The rats were sacrificed after each blood sampling, and lungs were removed for microscopic examination of the pathological changes of lungs which were scored and for determination of wet/dry lung weight ratio (W/D ratio) , myelopemxidase (MPO) activity (by spectrophotometry) and nuclear factor kappa B (NF-κB) p65 expression (using Western blot).Results Compared with group S, the serum TNF-α, IL-6 and IL-10 concentrations, W/D ratio, pathological scores, and MPO activity were significantly increased, and the expression of NF-κB p65 was up-regulated at T1-4 in Sep and SGB groups.Compared with group Sep, the serum TNF-α and IL-6 concentrations, W/D ratio, pathological scores, and MPO activity were significantly decreased, and the serum IL-10 concentrations were increased, and the expression of NF-κB p65 was down-regulated at T1-4 in group SGB.Conclusion SGB can attenuate acute lung injury in septic rats.