CAJ | 학술논문

目的评价线粒体钙单向转运体在大鼠肺缺血再灌注损伤中的作用.方法健康成年雄性Wistar大鼠30只,体重250～ 300 g,采用随机数字表法分为3组(n=10):假手术组(S组)、肺缺血再灌注组(I/R组)和线粒体钙单向转运体抑制剂钌红组(RR组).I/R组和RR组采用夹闭左肺门45 min,恢复灌注2h的方法制备肺缺血再灌注损伤模型;RR组于夹闭左肺门前1h时尾静脉注射钌红2.5 mg/kg.再灌注2h时,采集动脉血样0.5 ml,行血气分析,计算氧合指数;然后处死大鼠,取左肺上叶组织,计算肺组织湿重/干重(W/D)比值.结果与S组比较,I/R组和RR组氧合指数降低,肺组织W/D比值升高(P＜0 05);与I/R组比较,RR组氧合指数升高,肺组织W/D比值降低(P＜0.05).结论大鼠肺缺血再灌注损伤的机制与促进线粒体钙单向转运体的开放有关.
목적 평개선립체개단향전운체재대서폐결혈재관주손상중적작용.방법 건강성년웅성Wistar대서30지,체중250～ 300 g,채용수궤수자표법분위3조(n=10):가수술조(S조)、폐결혈재관주조(I/R조)화선립체개단향전운체억제제조홍조(RR조).I/R조화RR조채용협폐좌폐문45 min,회복관주2h적방법제비폐결혈재관주손상모형;RR조우협폐좌폐문전1h시미정맥주사조홍2.5 mg/kg.재관주2h시,채집동맥혈양0.5 ml,행혈기분석,계산양합지수;연후처사대서,취좌폐상협조직,계산폐조직습중/간중(W/D)비치.결과 여S조비교,I/R조화RR조양합지수강저,폐조직W/D비치승고(P＜0 05);여I/R조비교,RR조양합지수승고,폐조직W/D비치강저(P＜0.05).결론 대서폐결혈재관주손상적궤제여촉진선립체개단향전운체적개방유관.
Objective To evaluate the role of mitochondrial calcium uniporter in lung ischemiareperfusion (I/R) injury in rats.Methods Thirty healthy male Wistar rats, weighing 250-300 g, were randomly divided into 3 groups (n=10 each) using a random number table: sham operation group (group S) , group I/R and ruthenium red (mitochondrial calcium uniporter inhibitor) group (group RR).Lung I/R was produced by clamping the left pulmonary hilum for 45 min followed by 2 h reperfusion in I/R and RR groups.In addition, ruthenium red 2.5 mg/kg was injected via the tail vein at 1 h before occlusion of the left pulmonary hilum in RR group.Arterial blood samples were obtained at 2 h of reperfusion for blood gas analysis, and oxygenation index (PaO2/FiO2) was calculated.The rats were sacrificed, and then the lungs were removed for determination of wet/dry lung weight ratio (W/D ratio).Results Compared with group S, oxygenation index was significantly decreased, and W/D ratio was increased in I/R and RR groups.Oxygenation index was significantly higher, and W/D ratio was lower in group RR than in group I/R.Conclusion The mechanism underlying lung I/R injury is related to the opening of mitochondrial calcium uniporter in rats.