中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
Chinese Journal of Anesthesiology
2015年
7期
837-839
,共3页
右美托咪啶%二异丙酚%再灌注损伤%肠
右美託咪啶%二異丙酚%再灌註損傷%腸
우미탁미정%이이병분%재관주손상%장
Dexmedetomidine%Propofol%Reperfusion injury%Intestines
目的 比较右美托咪定与异丙酚对大鼠肠缺血再灌注损伤的影响.方法 SPF级健康成年雄性Wistar大鼠40只,2～3月龄,体重185 ～ 230 g,采用随机数字表法,将其分为4组(n=10):假手术组(S组)、肠缺血再灌注组(I/R组)、异丙酚组(P组)和右美托咪定组(D组).采用夹闭大鼠肠系膜上动脉20 min后恢复灌注的方法建立肠缺血再灌注损伤模型.P组和D组于缺血前20 min,分别经股静脉注射异丙酚10 mg/kg、右美托咪定5μg/kg.于再灌注2h时采集下腔静脉血样,测定血清二胺氧化酶(DAO)活性,光镜下观察肠黏膜上皮组织病理学结果,进行Chiu评分.结果 与S组比较,I/R组、P组和D组血清DAO活性和肠黏膜Chiu评分升高(P＜0.05).与I/R组比较,P组和D组血清DAO活性和肠黏膜Chiu评分降低(P＜0.05).与P组比较,D组血清DAO活性和肠黏膜Chiu评分降低(P＜0.05).结论 麻醉剂量右美托咪定减轻大鼠肠缺血再灌注损伤的效应优于异丙酚.
目的 比較右美託咪定與異丙酚對大鼠腸缺血再灌註損傷的影響.方法 SPF級健康成年雄性Wistar大鼠40隻,2～3月齡,體重185 ～ 230 g,採用隨機數字錶法,將其分為4組(n=10):假手術組(S組)、腸缺血再灌註組(I/R組)、異丙酚組(P組)和右美託咪定組(D組).採用夾閉大鼠腸繫膜上動脈20 min後恢複灌註的方法建立腸缺血再灌註損傷模型.P組和D組于缺血前20 min,分彆經股靜脈註射異丙酚10 mg/kg、右美託咪定5μg/kg.于再灌註2h時採集下腔靜脈血樣,測定血清二胺氧化酶(DAO)活性,光鏡下觀察腸黏膜上皮組織病理學結果,進行Chiu評分.結果 與S組比較,I/R組、P組和D組血清DAO活性和腸黏膜Chiu評分升高(P＜0.05).與I/R組比較,P組和D組血清DAO活性和腸黏膜Chiu評分降低(P＜0.05).與P組比較,D組血清DAO活性和腸黏膜Chiu評分降低(P＜0.05).結論 痳醉劑量右美託咪定減輕大鼠腸缺血再灌註損傷的效應優于異丙酚.
목적 비교우미탁미정여이병분대대서장결혈재관주손상적영향.방법 SPF급건강성년웅성Wistar대서40지,2～3월령,체중185 ～ 230 g,채용수궤수자표법,장기분위4조(n=10):가수술조(S조)、장결혈재관주조(I/R조)、이병분조(P조)화우미탁미정조(D조).채용협폐대서장계막상동맥20 min후회복관주적방법건립장결혈재관주손상모형.P조화D조우결혈전20 min,분별경고정맥주사이병분10 mg/kg、우미탁미정5μg/kg.우재관주2h시채집하강정맥혈양,측정혈청이알양화매(DAO)활성,광경하관찰장점막상피조직병이학결과,진행Chiu평분.결과 여S조비교,I/R조、P조화D조혈청DAO활성화장점막Chiu평분승고(P＜0.05).여I/R조비교,P조화D조혈청DAO활성화장점막Chiu평분강저(P＜0.05).여P조비교,D조혈청DAO활성화장점막Chiu평분강저(P＜0.05).결론 마취제량우미탁미정감경대서장결혈재관주손상적효응우우이병분.
Objective To compare the effects of dexmedetomidine and propofol on intestinal ischemia-reperfusion (I/R) injury in rats.Methods Forty male SPF Wistar rats, aged 2-3 months, weighing 185-230 g, were randomized into 4 groups (n=10 each) using a random number table: sham operation group (S group), intestinal I/R group (I/R group), propofol group (P group) and dexmedetomidine group (D group).Intestinal I/R was produced by occlusion of the superior mesenteric artery for 20 min followed by reperfusion.In P and D groups, propofol 10 mg/kg and dexmedetomidine 5 μg/kg were injected, respectively, via the femoral vein at 20 min before occlusion.At the end of 2 h reperfusion, the blood samples were collected from the inferior vena cava for determination of serum diamine oxidase (DAO) activity.A segment of the intestine of 5 cm in length was removed for microscopic examination with light microscope.The degree of damage to intestinal mucous membrane was scored according to Chiu.Results Compared to S group, the activity of DAO and Chiu' s score were significantly increased in I/R, P and D groups.Compared to I/R group, the activity of DAO and Chiu' s score were significantly decreased in P and D groups.Compared to P group, the activity of DAO and Chiu' s score were significantly decreased in group D.Conclusion Anesthetic dose of dexmedetomidine pretreatment reduces intestinal I/R injury in rats, and the effect is superior to that produced by propofol.
