CAJ | 학술논문

目的评价异丙酚对严重烫伤兔的肝保护效应.方法健康雄性新西兰兔20只,3～4月龄,体重2.3～2.5 kg.采用随机数字表法,将其分为2组(n=10):烫伤组(S组)和异丙酚组(P组).建立背部和臀部30％体表总面积Ⅲ度烫伤模型,P组烫伤后1h静脉注射异丙酚1.5 mg/kg,然后以4 mg· kg-1·h-1的速率静脉输注4h.S组以等容量的生理盐水替代异丙酚.于烫伤前(T1)、烫伤后1 h(T2)、异丙酚或生理盐水给药后6 h(T3)、12 h(T4)、24 h(T5)时采集颈内静脉血样,采用乳酸脱氢酶法检测血清谷丙转氨酶(ALT)活性,采用MDH法检测血清谷草转氨酶(AST)活性,采用酶联免疫吸附法检测血清TNF-α浓度.于T5时取肝脏,HE染色后观察病理学结果.结果与T1时比较,2组T25时血清ALT、AST活性和TNF-α浓度升高(P＜0.05或0.01).与T2时比较,T3-5时S组血清ALT、AST活性和TNF-α浓度升高,P组血清TNF-α浓度升高(P＜0.05或0.01),血清ALT和AST活性差异无统计学意义(P＞0.05).与S组比较,P组T3-5时血清ALT、AST活性和TNF-α浓度降低(P＜0.05或0.01),病理学损伤程度减轻.结论异丙酚对严重烫伤兔具有肝保护效应.
목적 평개이병분대엄중탕상토적간보호효응.방법 건강웅성신서란토20지,3～4월령,체중2.3～2.5 kg.채용수궤수자표법,장기분위2조(n=10):탕상조(S조)화이병분조(P조).건립배부화둔부30％체표총면적Ⅲ도탕상모형,P조탕상후1h정맥주사이병분1.5 mg/kg,연후이4 mg· kg-1·h-1적속솔정맥수주4h.S조이등용량적생리염수체대이병분.우탕상전(T1)、탕상후1 h(T2)、이병분혹생리염수급약후6 h(T3)、12 h(T4)、24 h(T5)시채집경내정맥혈양,채용유산탈경매법검측혈청곡병전안매(ALT)활성,채용MDH법검측혈청곡초전안매(AST)활성,채용매련면역흡부법검측혈청TNF-α농도.우T5시취간장,HE염색후관찰병이학결과.결과 여T1시비교,2조T25시혈청ALT、AST활성화TNF-α농도승고(P＜0.05혹0.01).여T2시비교,T3-5시S조혈청ALT、AST활성화TNF-α농도승고,P조혈청TNF-α농도승고(P＜0.05혹0.01),혈청ALT화AST활성차이무통계학의의(P＞0.05).여S조비교,P조T3-5시혈청ALT、AST활성화TNF-α농도강저(P＜0.05혹0.01),병이학손상정도감경.결론 이병분대엄중탕상토구유간보호효응.
Objective To evaluate the protective effect of propofol on liver in severely scalded rabbits.Methods Twenty healthy male New Zealand rabbits, aged 3-4 months, weighing 2.3-2.5 kg, were randomly divided into either scald group (group S, n =10) or propofol group (group P, n =10).Thirty percent of the total body surface was shaved chemically with 10％ sodium sulphate and then exposed to 98 ℃ water for 20 s to produce third degree thermal injury at the back and buttocks of anesthetized rats.In group P, propofol was injected at a dose of 1.5 mg/kg at 1 h after scald, followed by an infusion of 4 mg· kg-1 · h 1 for4 h.The equal volume of normal saline was given in groupS.Before scald (T1), at 1 h after scald (T2) , and at 6, 12 and 24 h after administration of propofol or normal saline (T3-5) , blood samples were taken from the internal jugular vein for determination of serum alanine aminotransferase (ALT) activity (by lactate dehydrogenase method), aspartate aminotransferase (AST) activity (by MDH), and tumor necrosis factor-alpha (TNF-α) concentrations (by enzyme linked immunosorbent assay).The rabbits were sacrificed at T5, and their livers were removed and cut into sections which were stained with haematoxylin and eosin and examined under microscope.Results The serum ALT and AST activities and TNF-α concentrations were significantly higher at T2-5 than at T1.Compared with the values at T2, the serum ALT and AST activities and TNF-α concentrations in group S and serum TNF-α concentrations in group P were significantly increased at T3-5, and no significant change was found in the serum ALT and AST activities at T3-5 in group P.Compared with group S, the serum ALT and AST activities and TNF-α concentrations were significantly decreased at T3 5, and the pathological changes were mitigated in group P.Conclusion Propofol provides protective effect on liver in severely scalded rabbits.