CAJ | 학술논문

目的评价脊髓神经元Mas相关基因受体C(MrgC)在小鼠骨癌痛维持中的作用.方法 SPF级雄性C3H/HeJ小鼠132只,8～10周龄,体重18～22 g.采用随机数字表法分为4组(n=33):假手术组(S组)、骨癌痛组(BCP组)、MrgC高选择性激动剂牛肾上腺素髓质8-22肽(BAM8-22)组(BAM组)和MrgC抗体组(MA组).采用右侧股骨远端骨髓腔接种含有2×105个NCTC 2472纤维肉瘤细胞的α-MEM 20 μl制备小鼠骨癌痛模型;S组注射20μl α-MEM.S组和BCP组在接种后第14天分别定时鞘内注射人工脑脊液5μl,BAM组和MA组分别注射BAM8-22 8 nmol/5μl及MrgC抗体5μl.连续给药7d,1次/d.分别于接种前1 d(T0)、给药前(T1)、接种后14、16、19、21 d(T2-5)时(首次给药前0.5 h及每次给药后2h)测定自发抬足次数(NSF)和机械缩足反应阈(MWT),每组于各时点随机取5只小鼠处死,取脊髓腰膨大,采用免疫荧光组织化学法检测脊髓神经元MrgC的表达.结果与S组比较,BCP组、BAM组和MA组T15时NSF增加,MWT降低,脊髓神经元MrgC表达上调(P＜0.05);与BCP组比较,BAM组T25时NSF降低,MWT升高,脊髓神经元MrgC表达上调(P＜0.05),MA组T2-5时NSF增加,MWT降低,脊髓神经元MrgC表达下调(P＜0.05).结论脊髓神经元MrgC参与了小鼠骨癌痛的维持.
목적 평개척수신경원Mas상관기인수체C(MrgC)재소서골암통유지중적작용.방법 SPF급웅성C3H/HeJ소서132지,8～10주령,체중18～22 g.채용수궤수자표법분위4조(n=33):가수술조(S조)、골암통조(BCP조)、MrgC고선택성격동제우신상선소수질8-22태(BAM8-22)조(BAM조)화MrgC항체조(MA조).채용우측고골원단골수강접충함유2×105개NCTC 2472섬유육류세포적α-MEM 20 μl제비소서골암통모형;S조주사20μl α-MEM.S조화BCP조재접충후제14천분별정시초내주사인공뇌척액5μl,BAM조화MA조분별주사BAM8-22 8 nmol/5μl급MrgC항체5μl.련속급약7d,1차/d.분별우접충전1 d(T0)、급약전(T1)、접충후14、16、19、21 d(T2-5)시(수차급약전0.5 h급매차급약후2h)측정자발태족차수(NSF)화궤계축족반응역(MWT),매조우각시점수궤취5지소서처사,취척수요팽대,채용면역형광조직화학법검측척수신경원MrgC적표체.결과 여S조비교,BCP조、BAM조화MA조T15시NSF증가,MWT강저,척수신경원MrgC표체상조(P＜0.05);여BCP조비교,BAM조T25시NSF강저,MWT승고,척수신경원MrgC표체상조(P＜0.05),MA조T2-5시NSF증가,MWT강저,척수신경원MrgC표체하조(P＜0.05).결론 척수신경원MrgC삼여료소서골암통적유지.
Objective To evaluate the role of spinal neuronal Mas-related gene receptor C (MrgC) in the maintenance of bone cancer pain (BCP) in mice.Methods A total of 132 SPF male C3H/HeJ mice, aged 8-10 weeks, weighing 18-22 g, were randomly divided into 4 groups (n=33 each) using a random number table: sham operation group (S group) , BCP group, bovine adrenal medulla peptide 8-22 (BAM8-22, a highly selective MrgC agonist) group (group BAM), and MrgC antibody group (group MA).BCP was produced by injecting α-MEM 20 μl containing 2×105NCTC2472 cells into the distal medullary cavity of right femur bone.While α-MEM 20 μl was injected only in group S.The artificial cerebrospinal fluid 5 μl was injected intrathecally in S and BCP groups, and BAM 8-22 8 nmol/5 μl and MrgC antibody 5 μl were injected intrathecally in BAM and MA groups, respectively, once a day for 7 consecutive days starting from the day 14 after inoculation of the tumor cells.At 1 day before inoculation (T0), before administration (T1) , and at 14, 16 19 and 21 days after inoculation (T2-5, at 0.5 h before the initial administration and 2 h after each administration) , the number of spontaneous flinches (NSF) and mechanical paw withdrawal threshold (MWT) were measured.Five animals selected from each group at each time point were sacrificed, and the lumbar enlargement segments of the spinal cord were removed for determination of MrgC expression in the spinal neurons (by immunofluorescence).Results Compared with group S, NSF was significantly increased, MWT was decreased, and the expression of MrgC was up-regulated at T1-5 in BCP, BAM and MA groups.Compared with group BCP, NSF was significantly decreased, MWT was increased, and the expression of MrgC was up-regulated at T2-5 in group BAM, and NSF was significantly increased, MWT was decreased, and the expression of MrgC was down-regulated at T2-5 in group MA.Conclusion Spinal neuronal MrgC is involved in the maintenance of BCP in mice.