中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
Chinese Journal of Anesthesiology
2015年
7期
879-882
,共4页
麻醉药,吸入%脊髓损伤%心肌%受体,钙敏感
痳醉藥,吸入%脊髓損傷%心肌%受體,鈣敏感
마취약,흡입%척수손상%심기%수체,개민감
Anesthetics,inhalation%Spinal cord injuries%Myocardium%Receptors,calcium-sensing
目的 评价七氟醚对高位脊髓损伤大鼠心肌钙敏感受体(CaSR)表达的影响.方法 健康雄性Wistar大鼠30只,体重250～ 300 g,采用随机数字表法分为3组:假手术组(S组,n=6)、高位脊髓损伤组(SCI组,n=12)和七氟醚组(Sev组,n=12).采用改良Allens法撞击脊髓制备高位脊髓损伤致大鼠心肌损伤模型.SCI组撞击后30 min持续吸入2 L/min纯氧30 min,Sev组吸入2％七氟醚30 min.于撞击后12和24 h(T12)时随机取6只大鼠,采集腹主动脉血样,测定血清心肌肌钙蛋白I(cTnI)浓度,随后处死大鼠,取心肌内壁组织,分别以实时荧光定量PCR法和Western blot法检测心肌CaSR及其mRNA的表达水平,透射电镜下观察心肌细胞超微结构.结果 与S组比较,SCI组和Sev组T12时血清cTnI浓度和心肌CaSR及其mRNA表达水平升高(P＜0.05);与SCI组比较,Sev组T12时血清cTnI浓度和心肌CaSR及其mRNA表达水平降低(P＜0.05).Sev组心肌细胞损伤程度较SCI组明显减轻.结论 七氟醚通过抑制CaSR表达减轻脊髓高位损伤大鼠心肌损伤.
目的 評價七氟醚對高位脊髓損傷大鼠心肌鈣敏感受體(CaSR)錶達的影響.方法 健康雄性Wistar大鼠30隻,體重250～ 300 g,採用隨機數字錶法分為3組:假手術組(S組,n=6)、高位脊髓損傷組(SCI組,n=12)和七氟醚組(Sev組,n=12).採用改良Allens法撞擊脊髓製備高位脊髓損傷緻大鼠心肌損傷模型.SCI組撞擊後30 min持續吸入2 L/min純氧30 min,Sev組吸入2％七氟醚30 min.于撞擊後12和24 h(T12)時隨機取6隻大鼠,採集腹主動脈血樣,測定血清心肌肌鈣蛋白I(cTnI)濃度,隨後處死大鼠,取心肌內壁組織,分彆以實時熒光定量PCR法和Western blot法檢測心肌CaSR及其mRNA的錶達水平,透射電鏡下觀察心肌細胞超微結構.結果 與S組比較,SCI組和Sev組T12時血清cTnI濃度和心肌CaSR及其mRNA錶達水平升高(P＜0.05);與SCI組比較,Sev組T12時血清cTnI濃度和心肌CaSR及其mRNA錶達水平降低(P＜0.05).Sev組心肌細胞損傷程度較SCI組明顯減輕.結論 七氟醚通過抑製CaSR錶達減輕脊髓高位損傷大鼠心肌損傷.
목적 평개칠불미대고위척수손상대서심기개민감수체(CaSR)표체적영향.방법 건강웅성Wistar대서30지,체중250～ 300 g,채용수궤수자표법분위3조:가수술조(S조,n=6)、고위척수손상조(SCI조,n=12)화칠불미조(Sev조,n=12).채용개량Allens법당격척수제비고위척수손상치대서심기손상모형.SCI조당격후30 min지속흡입2 L/min순양30 min,Sev조흡입2％칠불미30 min.우당격후12화24 h(T12)시수궤취6지대서,채집복주동맥혈양,측정혈청심기기개단백I(cTnI)농도,수후처사대서,취심기내벽조직,분별이실시형광정량PCR법화Western blot법검측심기CaSR급기mRNA적표체수평,투사전경하관찰심기세포초미결구.결과 여S조비교,SCI조화Sev조T12시혈청cTnI농도화심기CaSR급기mRNA표체수평승고(P＜0.05);여SCI조비교,Sev조T12시혈청cTnI농도화심기CaSR급기mRNA표체수평강저(P＜0.05).Sev조심기세포손상정도교SCI조명현감경.결론 칠불미통과억제CaSR표체감경척수고위손상대서심기손상.
Objective To evaluate the effect of sevoflurane on the expression of calcium-sensing receptor (CaSR) in the myocardium of rats with high-level spinal cord injury (SCI).Methods Thirty healthy male Wistar rats, weighing 250-300 g, were randomly divided into 3 groups using a random number table: sham operation group (group S, n = 6) , SCI group (n =12) and sevoflurane group (group Sev).SCI was induced in anesthetized rats by dropping a l0-g weight onto C7 spinal cord from 5.0 cm height falling freely inside a vertical hollow glass tube.Group SCI inhaled 2 L/min pure oxygen for 30 min, and group Sev inhaled 2％ sevoflurane for 30 min starting from 30 min after SCI.At 12 and 24 h after SCI (T1,2) , 6 rats were selected randomly, and blood samples from the abdominal aorta were drawn for determination of serum cardiac troponin I (cTnI) concentrations.The rats were then sacrificed, and myocardial specimens were obtained for determination of CaSR protein and mRNA expression (using fluorescent quantitative real-time reverse transcriptase-polymerase chain reaction or Western blot) and for examination of myocardial ultrastructure (with transmission electron microscope).Results Compared with group S, the serum cTnI concentrations and CaSR protein and mRNA expression were significantly increased at T1,2 in SCI and Sev groups.Compared with group SCI, the serum cTnI concentrations and CaSR protein and mRNA expression were significantly decreased at T1,2 in Sev group.The damage to myocardial cells was significantly reduced in group SCI compared with group Sev.Conclusion Sevoflurane reduces myocardial damage through inhibiting CaSR expression in the myocardium of rats with high-level SCI.
