中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
Chinese Journal of Anesthesiology
2015年
7期
876-878
,共3页
于冬梅%周锦%张铁铮%曹惠鹃
于鼕梅%週錦%張鐵錚%曹惠鵑
우동매%주금%장철쟁%조혜견
右美托咪啶%内毒素血症%肝
右美託咪啶%內毒素血癥%肝
우미탁미정%내독소혈증%간
Dexmedetomidine%Endotoxemia%Liver
目的 评价右美托咪定对内毒素血症大鼠急性肝损伤的影响.方法 成年雄性SD大鼠1 8只,3～4月龄,体重250～ 300 g,采用随机数字表法,将其分为3组(n=6):对照组(C组)、内毒素组(E组)和右美托咪定+内毒素组(D组).E组和D组经股静脉注射脂多糖(LPS)5 mg/kg.D组给予LPS后经15 min静脉输注右美托咪定负荷剂量7μg/kg,继以5μg·kg-1·h-1的速率输注6 h;E组和C组以等容量生理盐水替代.给药结束后,采集股静脉血样5 ml,采用ELISA法检测血清肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)浓度;采用IFCC推荐法测定血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性;取肝组织,电镜下观察病理学结果.结果 与C组比较,E组和D组血清ALT、AST活性及TNF-α、IL-1β浓度升高(P＜0.05);与E组比较,D组血清ALT和AST活性及TNF-α和IL-1β浓度降低(P＜0.05).E组病理学损伤明显,D组较E组病理学损伤程度减轻.结论 右美托咪定可减轻内毒素血症大鼠急性肝损伤,其机制与抑制全身炎性反应有关.
目的 評價右美託咪定對內毒素血癥大鼠急性肝損傷的影響.方法 成年雄性SD大鼠1 8隻,3～4月齡,體重250～ 300 g,採用隨機數字錶法,將其分為3組(n=6):對照組(C組)、內毒素組(E組)和右美託咪定+內毒素組(D組).E組和D組經股靜脈註射脂多糖(LPS)5 mg/kg.D組給予LPS後經15 min靜脈輸註右美託咪定負荷劑量7μg/kg,繼以5μg·kg-1·h-1的速率輸註6 h;E組和C組以等容量生理鹽水替代.給藥結束後,採集股靜脈血樣5 ml,採用ELISA法檢測血清腫瘤壞死因子-α(TNF-α)和白細胞介素-1β(IL-1β)濃度;採用IFCC推薦法測定血清穀丙轉氨酶(ALT)和穀草轉氨酶(AST)活性;取肝組織,電鏡下觀察病理學結果.結果 與C組比較,E組和D組血清ALT、AST活性及TNF-α、IL-1β濃度升高(P＜0.05);與E組比較,D組血清ALT和AST活性及TNF-α和IL-1β濃度降低(P＜0.05).E組病理學損傷明顯,D組較E組病理學損傷程度減輕.結論 右美託咪定可減輕內毒素血癥大鼠急性肝損傷,其機製與抑製全身炎性反應有關.
목적 평개우미탁미정대내독소혈증대서급성간손상적영향.방법 성년웅성SD대서1 8지,3～4월령,체중250～ 300 g,채용수궤수자표법,장기분위3조(n=6):대조조(C조)、내독소조(E조)화우미탁미정+내독소조(D조).E조화D조경고정맥주사지다당(LPS)5 mg/kg.D조급여LPS후경15 min정맥수주우미탁미정부하제량7μg/kg,계이5μg·kg-1·h-1적속솔수주6 h;E조화C조이등용량생리염수체대.급약결속후,채집고정맥혈양5 ml,채용ELISA법검측혈청종류배사인자-α(TNF-α)화백세포개소-1β(IL-1β)농도;채용IFCC추천법측정혈청곡병전안매(ALT)화곡초전안매(AST)활성;취간조직,전경하관찰병이학결과.결과 여C조비교,E조화D조혈청ALT、AST활성급TNF-α、IL-1β농도승고(P＜0.05);여E조비교,D조혈청ALT화AST활성급TNF-α화IL-1β농도강저(P＜0.05).E조병이학손상명현,D조교E조병이학손상정도감경.결론 우미탁미정가감경내독소혈증대서급성간손상,기궤제여억제전신염성반응유관.
Objective To evaluate the effect of dexmedetomidine on acute liver injury in rats with endotoxemia.Methods Eighteen adult male Sprague-Dawley rats, aged 3-4 months, weighing 250-300 g, were randomly divided into 3 groups (n=6 each) using a random number table: control group (group C), endotoxin group (group E), and dexmedetomidine group (group D).In E and D groups, lipopo-lysaccharide 5 mg/kg was injected via the femoral vein of rats anesthetized with chloral hydrate.In group D, dexmedetomidine was infused with a 7 μg/kg loading bolus over 15 min after injection of lipopolysaccharide, followed by a 6 h continuous infusion of 5 μg · kg-1 · h-1.The equal volume of normal saline was given instead in E and C groups.After the end of administration, blood samples from the femoral vein were drawn for determination of tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) concentrations in serum (by using enzyme-linked immunosorbent assay), and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in serum (using the International Federation of Clinical Chemistry and Laboratory Medicine reference procedures).Liver specimens were obtained for examination of pathologic changes with electron microscope.Results Compared with group C, the serum ALT and AST activities and TNF-α and IL-lβ concentrations were significantly increased in E and D groups.Compared with group E, the serum ALT and AST activities and TNF-α and IL-1β concentrations were significantly decreased in group D.The pathologic changes of livers were obvious in group E, and were significantly reduced in group D compared with group E.Conclusion Dexmedetomidine can alleviate acute liver injury in rats with endotoxemia, and the underlying mechanism is associated with inhibition of systemic inflammatory responses.
