CAJ | 학술논문

目的：探讨调气消积汤对人肺腺癌 A549细胞增殖与凋亡的影响。方法：以不同剂量的调气消积汤及顺铂制作含药血清并分别作用于 A549细胞，MTT 法检测细胞的增殖能力，TUNEL 原位法染色标记凋亡细胞，计算凋亡率。结果：调气消积汤低剂量组在不同时间段对 A549细胞增殖没有明显影响，中、高剂量组及顺铂组对 A549细胞增殖有明显的抑制作用，与空白对照组相比，差异有统计学意义（P ＜0.05或 P ＜0.01）。调气消积汤各剂量组凋亡细胞的数量与剂量呈正相关，凋亡率与空白对照组相比，差异显著（ P ＜0.01），顺铂组绿色荧光强度最大。结论：调气消积汤可剂量依赖性抑制 A549细胞增殖并诱导其凋亡。
목적：탐토조기소적탕대인폐선암 A549세포증식여조망적영향。방법：이불동제량적조기소적탕급순박제작함약혈청병분별작용우 A549세포，MTT 법검측세포적증식능력，TUNEL 원위법염색표기조망세포，계산조망솔。결과：조기소적탕저제량조재불동시간단대 A549세포증식몰유명현영향，중、고제량조급순박조대 A549세포증식유명현적억제작용，여공백대조조상비，차이유통계학의의（P ＜0.05혹 P ＜0.01）。조기소적탕각제량조조망세포적수량여제량정정상관，조망솔여공백대조조상비，차이현저（ P ＜0.01），순박조록색형광강도최대。결론：조기소적탕가제량의뢰성억제 A549세포증식병유도기조망。
Objective:To approach the effect of Tiaoqixiaoji Decoction(TQXJ)on the proliferation and apoptosis of human lung adenocarcinoma A549 cells. Methods:A549 cells after treatment with different concentrations of TQXJ serum were observed. The proliferation of A549 cells were detected by MTT method,and the apoptosis were detected by TUNEL method. Results:The proliferation of A549 cells were inhibited obviously in median and high dose TQXJ groups,there was statistical difference compared with the blank group(P < 0. 05 or P < 0. 01). Conclusion:The Tiaoqixiaoji Decoction can inhibit the proliferation in dose - dependent manner and induce apoptosis of A549 cells.