CAJ | 학술논문

目的：探讨 FLOT2在肾细胞癌细胞株中的表达，并观察小干扰 RNA 沉默 FLOT2基因对肾细胞癌786- O 细胞增殖能力的影响。方法：将化学合成的 FLOT2的小干扰 siRNA 用脂质体 Lipofectamine2000转染至肾细胞癌786- O 细胞；分别用 RT - PCR、Western blot 方法检测细胞转染前后 FLOT2以及细胞增殖相关因子 CCND1的表达。CCK -8与克隆形成实验检测其增殖情况。结果：转染 FLOT2- siRNA 的肾细胞癌786-O 细胞，RT - PCR、Western blot 检测显示 FLOT2在基因及蛋白水平明显下调（P ＜0.05）；与对照组比较786-O 细胞增殖能力明显下降（P ＜0.05），细胞增殖相关因子 CCND1明显下调（P ＜0.05）。结论：FLOT2在肾细胞癌细胞系中高表达，采用特异性 FLOT2- siRNA 能够显著降低 FLOT2和 CCND1表达。干扰 FLOT2表达能显著抑制786- O 细胞的增殖，FLOT2基因可能参与了肾细胞癌的生物学行为。
목적：탐토 FLOT2재신세포암세포주중적표체，병관찰소간우 RNA 침묵 FLOT2기인대신세포암786- O 세포증식능력적영향。방법：장화학합성적 FLOT2적소간우 siRNA 용지질체 Lipofectamine2000전염지신세포암786- O 세포；분별용 RT - PCR、Western blot 방법검측세포전염전후 FLOT2이급세포증식상관인자 CCND1적표체。CCK -8여극륭형성실험검측기증식정황。결과：전염 FLOT2- siRNA 적신세포암786-O 세포，RT - PCR、Western blot 검측현시 FLOT2재기인급단백수평명현하조（P ＜0.05）；여대조조비교786-O 세포증식능력명현하강（P ＜0.05），세포증식상관인자 CCND1명현하조（P ＜0.05）。결론：FLOT2재신세포암세포계중고표체，채용특이성 FLOT2- siRNA 능구현저강저 FLOT2화 CCND1표체。간우 FLOT2표체능현저억제786- O 세포적증식，FLOT2기인가능삼여료신세포암적생물학행위。
Objective:To investigate the effects of flotillin - 2(FLOT2)gene expression silencing on the prolifera-tion in vitro inhuman renal cell carcinoma cell line 786 - O. Methods:Chemically synthesized siRNA targeting FLOT2 (FLOT2 - siRNA)was transfected into human renal cell carcinoma cell line 786 - O cells by lipofectamine 2 000. The expressions of FLOT2 mRNA were detected by PCR. The proteins of FLOT2 and CCND1 and after cell transfec-tion were detected by Western blot. The effects of altered expression of FLOT2 on the cell proliferation were measured by CCK - 8 and colony formation assay. Results:After cell transfection,the FLOT2 mRNA expressions and proteins were significantly decreased(P < 0. 05). The abilities of proliferation were inhibited and level of CCND1 was down -regulated(P < 0. 05). Conclusion:FLOT2 expression in renal clear cell carcinoma is higher than in normal renal cells,FLOT2 - siRNA can down - regulate the expression of FLOT2 protein in 786 - O cells,inhibit the proliferation and colony formation of 786 - O cells effectively,which suggest that FLOT2 may be involved in the malignant behavior of renal cell carcinoma.