CAJ | 학술논문

目的：探讨蜗牛酶酶解毛蕊异黄酮苷转化生成毛蕊异黄酮的最佳转化条件和方法。方法采用恒温摇床，将蜗牛酶溶于合适温度的水中，加入用DMSO溶解的苷，边加边搅拌，加完后反应30 min，最后用乙腈终止反应。通过反复重结晶得到所需要的苷元。结果最佳转化条件为，当蜗牛酶与毛蕊异黄酮苷的比例为1：2时，加入200 mL的保温35~37℃水中，待蜗牛酶溶解均匀后，再加入毛蕊异黄酮苷（溶于1 mL的DMSO中），pH维持5~8，孵化0.5 h后，用乙腈等量终止反应。结论该法能有效使毛蕊异黄酮苷转化为纯度大于98%的毛蕊异黄酮，方法简单，易于操作。
목적：탐토와우매매해모예이황동감전화생성모예이황동적최가전화조건화방법。방법채용항온요상，장와우매용우합괄온도적수중，가입용DMSO용해적감，변가변교반，가완후반응30 min，최후용을정종지반응。통과반복중결정득도소수요적감원。결과최가전화조건위，당와우매여모예이황동감적비례위1：2시，가입200 mL적보온35~37℃수중，대와우매용해균균후，재가입모예이황동감（용우1 mL적DMSO중），pH유지5~8，부화0.5 h후，용을정등량종지반응。결론해법능유효사모예이황동감전화위순도대우98%적모예이황동，방법간단，역우조작。
Objective To study the best best conditions and methods to conduct the transformation of calycosin-7-O-β- D-gluco-side into calycosin using the enzymatic hydrolysis with snailase. Methods The snailase was dissolved in a suitable water in constant temperature shaking table and then added dimethyl sulfoxide ( DMSO ) which calycosin-7-O-β- D-glucoside was dissolved in, kept the reaction for 30 min, and finally terminated the reaction with acetonitrile. Results When the snailase and calycosin-7-O-β- D-glucoside were in the ratio of 1:2, added with 200 mL water ( keeping in 35-37 ℃) , added calycosin-7-O-β- D-glucoside that was dissolved in 1 mL of DMSO, and the pH was maintained at between 5-8, then quenched with an equal amount of acetonitrile af-ter incubating 0. 5 h. Conclusion This method can easily transform the calycosin-7-O-β- D-glucose into calycosin ( ﹥ 98%) , and the operating process is simple and effective.