医学信息
醫學信息
의학신식
Medical Information
2015年
40期
83-84
,共2页
黄远帅%周维鑫%罗彬瑞%郭天虹
黃遠帥%週維鑫%囉彬瑞%郭天虹
황원수%주유흠%라빈서%곽천홍
乙肝标志物%HBV DNA%时间分辨荧光分析%实时荧光定量PCR
乙肝標誌物%HBV DNA%時間分辨熒光分析%實時熒光定量PCR
을간표지물%HBV DNA%시간분변형광분석%실시형광정량PCR
HBV marker%HBV DNA%TRFIA%Real-time PCR
目的：探讨乙肝标志物不同血清模式与HBV-DNA结果者之间的关系及临床意义。方法收集2014年6月~8月乙肝患者标本251例,采用时间分辨免疫方法(TRFIA)和实时荧光定量PCR方法(FQ-PCR)分别检测乙肝病毒血清标记物和病毒DNA,比较它们之间的关系。结果 HBsAg、HBeAg、HBcAb阳性组79例,HBV DNA定量阳性共72例；HBsAg、HBeAb、HBcAb阳性组101例,HBV DNA定量阳性63例,HBV DNA定量阴性38例；HBcAb阳性,其余全阴组35例,HBV DNA定量阳性19例,HBV DNA定量阴性14例；五项全阴组36例, HBV DNA定量阴性36例。HBV DNA在HBcAb阳性组与HBcAb阴性组间相比,有显著的统计学差异(﹤0.05)。FQ-PCR法和TRFIA法诊断乙肝的敏感性分别为71.6%和100%,符合率为85.8%。结论 FQ-PCR法和TRFIA法在HBcAg阳性组有良好的相关性,两种方法分别检测乙肝病毒感染机体不同状态,它们之间能互为补充,不能相互替代。
目的：探討乙肝標誌物不同血清模式與HBV-DNA結果者之間的關繫及臨床意義。方法收集2014年6月~8月乙肝患者標本251例,採用時間分辨免疫方法(TRFIA)和實時熒光定量PCR方法(FQ-PCR)分彆檢測乙肝病毒血清標記物和病毒DNA,比較它們之間的關繫。結果 HBsAg、HBeAg、HBcAb暘性組79例,HBV DNA定量暘性共72例；HBsAg、HBeAb、HBcAb暘性組101例,HBV DNA定量暘性63例,HBV DNA定量陰性38例；HBcAb暘性,其餘全陰組35例,HBV DNA定量暘性19例,HBV DNA定量陰性14例；五項全陰組36例, HBV DNA定量陰性36例。HBV DNA在HBcAb暘性組與HBcAb陰性組間相比,有顯著的統計學差異(﹤0.05)。FQ-PCR法和TRFIA法診斷乙肝的敏感性分彆為71.6%和100%,符閤率為85.8%。結論 FQ-PCR法和TRFIA法在HBcAg暘性組有良好的相關性,兩種方法分彆檢測乙肝病毒感染機體不同狀態,它們之間能互為補充,不能相互替代。
목적：탐토을간표지물불동혈청모식여HBV-DNA결과자지간적관계급림상의의。방법수집2014년6월~8월을간환자표본251례,채용시간분변면역방법(TRFIA)화실시형광정량PCR방법(FQ-PCR)분별검측을간병독혈청표기물화병독DNA,비교타문지간적관계。결과 HBsAg、HBeAg、HBcAb양성조79례,HBV DNA정량양성공72례；HBsAg、HBeAb、HBcAb양성조101례,HBV DNA정량양성63례,HBV DNA정량음성38례；HBcAb양성,기여전음조35례,HBV DNA정량양성19례,HBV DNA정량음성14례；오항전음조36례, HBV DNA정량음성36례。HBV DNA재HBcAb양성조여HBcAb음성조간상비,유현저적통계학차이(﹤0.05)。FQ-PCR법화TRFIA법진단을간적민감성분별위71.6%화100%,부합솔위85.8%。결론 FQ-PCR법화TRFIA법재HBcAg양성조유량호적상관성,량충방법분별검측을간병독감염궤체불동상태,타문지간능호위보충,불능상호체대。
Objective To investigate the relationship and clinical significance of serum HBV markers in dif erent pat erns and HBV-DNA results among them.Methods From June 2014 to August 2014 specimens of 251 cases of hepatitis B patients,the use of time-resolved immunoassay (TRFIA)and real-time quantitative PCR (FQ-PCR)were used to detect hepatitis B virus serum markers and viral DNA,compare them Relationship between.Results HBsAg,HBeAg,HBcAb 79 cases positive,HBV DNA quantification positive total 72 cases;HBsAg,HBeAb,101 HBcAb positive patients,HBV DNA quantification positive 63 cases,HBV DNA quantification negative 38 cases;HBcAb positive,the rest overcast group of 35 patients,HBV DNA quantification positive 19 cases,HBV DNA quantification negative 14 cases;five ful female group of 36 patients,HBV DNA quantification negative 36 cases.HBV DNA in HBcAb between positive and negative HBcAb compared with a statistical y significant dif erence ( ﹤0.05).FQ-PCR method and sensitivity TRFIA diagnosis of hepatitis B were 71.6%and 100%,the rate was 85.8%.Conclusion FQ-PCR method and TRFIA method HBcAg positive group has a good cor elation between the two methods were used to detect hepatitis B virus infection in dif erent states of the body,can complement each other between them,can not replace each other.
