CAJ | 학술논문

目的了解北京市房山区腹泻病人中轮状病毒和人杯状病毒的感染情况.方法 2011年4月-2013年3月收集北京市两家医院肠道门诊腹泻患者便标本243份,采用ELISA检测轮状病毒抗原,采用一步法RT-PCR对人杯状病毒核酸进行检测.结果 60份样本病毒检测阳性,轮状病毒检出率为10.3％(25/243),人杯状病毒检出率为14.4％(35/243).60岁以上患者轮状病毒阳性检出率为12.1％(4/33),高于其他年龄组.12月份到次年3月份为轮状病毒腹泻的高发季节,人杯状病毒腹泻的季节性特征不明显.结论轮状病毒和人杯状病毒是北京市房山区肠道门诊腹泻患者的重要病原体.
목적 료해북경시방산구복사병인중륜상병독화인배상병독적감염정황.방법 2011년4월-2013년3월수집북경시량가의원장도문진복사환자편표본243빈,채용ELISA검측륜상병독항원,채용일보법RT-PCR대인배상병독핵산진행검측.결과 60빈양본병독검측양성,륜상병독검출솔위10.3％(25/243),인배상병독검출솔위14.4％(35/243).60세이상환자륜상병독양성검출솔위12.1％(4/33),고우기타년령조.12월빈도차년3월빈위륜상병독복사적고발계절,인배상병독복사적계절성특정불명현.결론 륜상병독화인배상병독시북경시방산구장도문진복사환자적중요병원체.
Objective To understand the epidemiological characteristics of rotavirus and human calicivirus (HuCV) infection among patients with diarrhea in Fangshan district.Methods A total of 243 stool samples were collected from diarrhea patients at 2 sentinel hospitals.Rotavirus antigen was detected by ELISA, and the nncleic acid of HuCV was detected by one step reverse transcriptase polymerase chain reaction (RT-PCR).Results Sixty samples were virus-positive, and the detection rates of rotavirus and HuCV were 10.3％ (25/243), 14.4％ (35/243), respectively.The highest detection rate of rotavirus (12.1％, 4/33) was found among patients over 60 years old.The rotavirus infections were prevalent during DecemberMarch, and the seasonality of HuCV infections was not significant.Conclusions Rotavirus and HuCV were the important causes of diarrhea in Fangshan district, Beijing.