CAJ | 학술논문

目的：优化核苷磷酸化酶的固定化工艺，并对固定化酶的性质进行研究。方法通过正交试验优化核苷磷酸化酶（包括嘌呤和嘧啶核苷磷酸化酶）固定化条件，并进一步对固定化核苷磷酸化酶的最适pH、温度及其稳定性进行研究。结果固定化酶条件为pH＝8．0，温度为25℃，蛋白载量为20 mg／g载体，固定化时间为6 h；固定化核苷磷酸化酶的最适pH＝7．5，最适温度为40℃，固酶连续使用20批次酶活力无明显损失。结论固定化核苷磷酸化酶稳定可靠，适合应用于工业化生产。
목적：우화핵감린산화매적고정화공예，병대고정화매적성질진행연구。방법통과정교시험우화핵감린산화매（포괄표령화밀정핵감린산화매）고정화조건，병진일보대고정화핵감린산화매적최괄pH、온도급기은정성진행연구。결과고정화매조건위pH＝8．0，온도위25℃，단백재량위20 mg／g재체，고정화시간위6 h；고정화핵감린산화매적최괄pH＝7．5，최괄온도위40℃，고매련속사용20비차매활력무명현손실。결론고정화핵감린산화매은정가고，괄합응용우공업화생산。
Objective To optimize the immobilization process of the nucleoside phosphorylase and research on its property.Methods The immobilization process of the nucleoside phosphorylase(including purine and pyrimidine nucleoside phosphorylase)was optimized by orthogonal test,and took further study of the finest pH,temperature and stability of immobilized nucleoside phosphorylase.Results The finest condition of immobilized enzyme was pH=8.0,25 ℃,20 mg/g protein loads and immobilize for 6 h;the finest condition for the immobilized nucleoside phosphorylase to catalyze was pH=7.5 and 40 ℃.There was no significant loss of activity of the enzyme after use for catalyzing 20 batches consecutively.Conclusion The immobilized nucleoside phosphorylase is stable and reliable,which is suit-able to application in industrial production.