中国药业
中國藥業
중국약업
China Pharmaceuticals
2015年
20期
79-80
,共2页
定喘止咳糖浆%高效液相色谱法%麻黄碱%伪麻黄碱
定喘止咳糖漿%高效液相色譜法%痳黃堿%偽痳黃堿
정천지해당장%고효액상색보법%마황감%위마황감
Dingchuan Zhike Syrup%HPLC%ephedrine hydrochloride%pseudoephedrine hydrochloride
目的：建立测定喘止咳糖浆含量的高效液相色谱（HPLC）法。方法色谱柱为Ultimate XB-C18柱(250 mm ×4．6 mm，5μm)，流动相为0．02 mol／L磷酸二氢钠缓冲液（pH＝3．0，0．4％的三乙胺）-甲醇（96∶4），流速0．9 mL／min，柱温30℃，检测波长210 nm。结果盐酸麻黄碱质量浓度在1．9344~30．9500μg／mL(r＝1．0000)与峰面积线性关系良好，平均回收率为99．50％；盐酸伪麻黄碱质量浓度在2．4813~39．7000μg／mL(r＝0．9999)与峰面积线性关系良好，平均回收率为96．57％（n＝5）。结论该方法简便、准确，重复性好，适用于定喘止咳糖浆的质量控制。
目的：建立測定喘止咳糖漿含量的高效液相色譜（HPLC）法。方法色譜柱為Ultimate XB-C18柱(250 mm ×4．6 mm，5μm)，流動相為0．02 mol／L燐痠二氫鈉緩遲液（pH＝3．0，0．4％的三乙胺）-甲醇（96∶4），流速0．9 mL／min，柱溫30℃，檢測波長210 nm。結果鹽痠痳黃堿質量濃度在1．9344~30．9500μg／mL(r＝1．0000)與峰麵積線性關繫良好，平均迴收率為99．50％；鹽痠偽痳黃堿質量濃度在2．4813~39．7000μg／mL(r＝0．9999)與峰麵積線性關繫良好，平均迴收率為96．57％（n＝5）。結論該方法簡便、準確，重複性好，適用于定喘止咳糖漿的質量控製。
목적：건립측정천지해당장함량적고효액상색보（HPLC）법。방법색보주위Ultimate XB-C18주(250 mm ×4．6 mm，5μm)，류동상위0．02 mol／L린산이경납완충액（pH＝3．0，0．4％적삼을알）-갑순（96∶4），류속0．9 mL／min，주온30℃，검측파장210 nm。결과염산마황감질량농도재1．9344~30．9500μg／mL(r＝1．0000)여봉면적선성관계량호，평균회수솔위99．50％；염산위마황감질량농도재2．4813~39．7000μg／mL(r＝0．9999)여봉면적선성관계량호，평균회수솔위96．57％（n＝5）。결론해방법간편、준학，중복성호，괄용우정천지해당장적질량공제。
Objective To formulate the HPLC method of content determination of the Dingchuan Zhike Syrup.Methods The chromatog-raphy:Ultimate-XB C18 column(250 mm ×4.6 mm,5 μm);Mobile phase:methanol-0.02 mol/L sodium dihydrogen phosphate buffer (pH=3.0,containing 0.4% of triethylamine)(96∶4);flow rate:0.9 mL/min;column temperature:30 ℃;detection wavelength:210 nm. Results Ephedrine hydrochloride was linear in the range of 1.934 4-30.950 0 μg/mL(r=1.000 0),the average recovery was 99.50%.Pseudoephedrine hydrochloride was linear in the range of 2.481 3~39.700 0 μg/mL(r=0.999 9),the average recovery was 96.57%(n=5 ).Conclusion The method is simple and accurate,it also has good reproducibility.This method can be applied to con-trol the quality of the dingchuan zhike syrup.
