CAJ | 학술논문

以5-氯水杨醛,4-溴甲基苯硼酸,2-氨基-5-氯苯甲酰胺为原料合成了一种基于ELF-97的新型H2 O2荧光探针P1,并用1 H NMR,13 C NMR和HRMS对其结构进行了表征。此探针在0.02 mol/L PBS-DMSO (9：1, V/V, pH=7.4)的溶液中,在360 nm激发光激发下,最大发射波长为425 nm,荧光微弱；加入H2 O2后,相同的激发波长下,425 nm发射峰消失,同时在515 nm处出现强的荧光发射峰。515 nm处荧光强度与H2 O2的浓度呈动态学相关性,线性响应范围为5~45μmol/L,检出限为0.1μmol/L,牛奶中检测H2 O2的回收率在94.0%~106.0%之间。探针P1对H2 O2的快速检测具有潜在的应用价值。
이5-록수양철,4-추갑기분붕산,2-안기-5-록분갑선알위원료합성료일충기우ELF-97적신형H2 O2형광탐침P1,병용1 H NMR,13 C NMR화HRMS대기결구진행료표정。차탐침재0.02 mol/L PBS-DMSO (9：1, V/V, pH=7.4)적용액중,재360 nm격발광격발하,최대발사파장위425 nm,형광미약；가입H2 O2후,상동적격발파장하,425 nm발사봉소실,동시재515 nm처출현강적형광발사봉。515 nm처형광강도여H2 O2적농도정동태학상관성,선성향응범위위5~45μmol/L,검출한위0.1μmol/L,우내중검측H2 O2적회수솔재94.0%~106.0%지간。탐침P1대H2 O2적쾌속검측구유잠재적응용개치。
A novel 2-( 2’-hydroxy-5’-chlorophenyl )-6-chloro-4 ( 3 H )-quinazolinone ( ELF-97 )-based fluorescent probe (P1) for hydrogen peroxide was prepared from 5-chlorosalicylaldehyde, 4-(bromomethyl) phenylboronic acid and 2-amino-5-chlorobenzamide, and its structure was characterized by 1 H NMR, 13 C NMR and HRMS. Weak fluorescence intensity was observed at 425nm when the solution of probe P1 was excited by 360 nm UV light. After addition of H2 O2 , however, emission peak at 425 nm disappeared while strong peak emission at 515 nm appeared with the same excitation wavelength ( 360 nm ) . The fluorescence intensity at 515 nm was dependent on the concentration of H2 O2 in the linear response range of 5-45 μmol/L. The detection limit of H2O2 was 0. 1 μmol/L (S/N=3) and the recovery rates of added H2O2 in milk were in the range of 94 . 0%-106 . 0%. Probe P1 was potential to become a useful tool for rapid detection of hydrogen peroxide.