CAJ | 학술논문

建立了植物油中15+1种欧盟优控多环芳烃的在线固相萃取-高效液相色谱-紫外/荧光( online-SPE-HPLC-UV/FLD)检测分析方法。食用油样品经异丙醇稀释定容并过滤,固相萃取柱ChromSpher Pi (80 mm×3 mm)在线净化,反相C18 PAH专用柱(250 mm×4.6 mm i. d,5μm)分离,异丙醇、乙腈和水为流动相,梯度洗脱,紫外(220 nm)和荧光检测器检测。结果表明,15+1种多环芳烃在相应的线性范围内峰面积与质量浓度之间线性良好(R2>0.99)；检出限为0.03~12.23μg/kg；3个水平加标回收率为65.3%~110.5%；相对标准偏差为0.1%~9.8%。
건립료식물유중15+1충구맹우공다배방경적재선고상췌취-고효액상색보-자외/형광( online-SPE-HPLC-UV/FLD)검측분석방법。식용유양품경이병순희석정용병과려,고상췌취주ChromSpher Pi (80 mm×3 mm)재선정화,반상C18 PAH전용주(250 mm×4.6 mm i. d,5μm)분리,이병순、을정화수위류동상,제도세탈,자외(220 nm)화형광검측기검측。결과표명,15+1충다배방경재상응적선성범위내봉면적여질량농도지간선성량호(R2>0.99)；검출한위0.03~12.23μg/kg；3개수평가표회수솔위65.3%~110.5%；상대표준편차위0.1%~9.8%。
A novel method was developed for the determination of the 15+1 European priority polycyclic aromatic hydrocarbons in edible oil by online solid-phase extraction coupled with high performance liquid chromatography-ultraviolet/ fluorescence detection ( online-SPE-HPLC-UV/FL-D ) . The edible oil samples were diluted with isopropyl alcohol, and then filtered. The online extraction was performed on a solid phase extraction ChromSpher Pi column (80 mmí3 mm) and the separation was carried out on a C18 reversed-phase PAH column (250 mmí4. 6 mm i. d, 5μm) using ultraviolet detection at 220 nm and fluorescence detection. Isopropyl alcohol, acetonitrile and water were served as mobile phase in gradient elution. The results showed good linearity for the 15+1 polycyclic aromatic hydrocarbons with all the correlation coefficients (R2)>0. 99. The limits of detection ( LODs ) were between 0. 03 and 12. 23 μg/kg. The recoveries of the sixteen components in the three levels of spiked samples were in the range of 65 . 3%-110 . 5% with the relative standard deviation (RSD, n=6) from 0. 1% to 9. 8%.