CAJ | 학술논문

基于目标物循环放大策略及苝酐衍生物功能化纳米探针,构建了超灵敏的电致化学发光( ECL)传感器用于Pb2+检测。将发卡型DNA底物链通过分子自组装固载于沉积纳米金的玻碳电极表面,当目标物Pb2+及脱氧核酶( DNAzyme)存在时,底物链被剪切,在电极上留下单链DNA,同时释放出的Pb2+和DNAzyme可进入下一个循环,继续对未剪切的底物链进行剪切。剪切得到的单链DNA可与辅助探针H1的一端杂交, H1的另一端可与标记有苝酐衍生物功能化空心纳米金的发夹探针H2杂交。随着Pb2+浓度的增加,更多的信号探针被捕获,使得电极上产生的ECL信号逐渐增强。在Pb2+不存在时,底物链不能被剪切,信号探针不能被捕获,ECL信号低。本传感器在1×10-12~1×10-6 mol/L的浓度范围内对Pb2+有良好的线性响应,检出限为1×10-12 mol/L(S/N=3)。传感器对常见的金属离子表现出良好的选择性。
기우목표물순배방대책략급패항연생물공능화납미탐침,구건료초령민적전치화학발광( ECL)전감기용우Pb2+검측。장발잡형DNA저물련통과분자자조장고재우침적납미금적파탄전겁표면,당목표물Pb2+급탈양핵매( DNAzyme)존재시,저물련피전절,재전겁상류하단련DNA,동시석방출적Pb2+화DNAzyme가진입하일개순배,계속대미전절적저물련진행전절。전절득도적단련DNA가여보조탐침H1적일단잡교, H1적령일단가여표기유패항연생물공능화공심납미금적발협탐침H2잡교。수착Pb2+농도적증가,경다적신호탐침피포획,사득전겁상산생적ECL신호축점증강。재Pb2+불존재시,저물련불능피전절,신호탐침불능피포획,ECL신호저。본전감기재1×10-12~1×10-6 mol/L적농도범위내대Pb2+유량호적선성향응,검출한위1×10-12 mol/L(S/N=3)。전감기대상견적금속리자표현출량호적선택성。
Based on target cycling amplification and 3 ,4 ,9 ,10-perylenetetracarboxylic dianhydride derivative functionalized singal probe, an ultrasensitive electrochemiluminescence ( ECL) sensor was designed for the detection of lead ions. The hairpin substrate DNA was immobilized on the electrode through molecular self-assembly. In the presence of Pb2+and DNAzyme, the substrate was cleaved with single strand DNA fragments left on the electrode surface. Meanwhile, the target and DNAzyme was released for another cleaving circularly. As a result, the single strand DNA fragments hybridized with the assist hairpin probe H1, which leaded to the fabrication of H2 labeled with the 3 , 4 , 9 , 10-perylenetetracarboxylic dianhydride derivative functionalized hollow gold nanoparticles. With the increasing concentration of Pb2+, much more signal probe was been captured and the ECL signal of the biosensor in peroxydisulfate ( S2 O2-8 ) solution would increase. An ECL assay demonstrates that the sensor has a good linear response to Pb2+ concentration in the range of 1í10-12 mol/L-1í10-6 mol/L, with a detection limit of 1í10-12 mol/L. The fabricated sensor shows good selectivity toward Pb2+against other common metal ions.