CAJ | 학술논문

目的：检测苯扎贝特干预后小鼠肝脏 SR-B1mRNA 表达，探讨苯扎贝特干预后对小鼠肝脏 B 族 I 型清道夫受体（SR-B1）mRNA 的影响。方法：35只 C57BL/6小鼠随机分为5组，分别给予普通饲料、不同剂量的苯扎贝特（0.1%、0.25%、0.5%、0.75%）添加普通饲料喂养4周后，应用逆转录聚合酶链反应测定小鼠肝脏 SR-B1mRNA 表达。结果：不同剂量苯扎贝特（0.1%、0.25%、0.5%、0.75%）干预后小鼠肝脏 SR-BImRNA 的表达分别为0.22±0.07、0.53±0.04、0.84±0.01、0.92±0.05，与对照组0.11±0.04比较，苯扎贝特明显增加小鼠肝脏 SR-BImRNA 的表达。结论：苯扎贝特能够上调肝脏 SR-B1mRNA 表达，促进体内胆固醇逆转运，利于动脉粥样硬化防治。
목적：검측분찰패특간예후소서간장 SR-B1mRNA 표체，탐토분찰패특간예후대소서간장 B 족 I 형청도부수체（SR-B1）mRNA 적영향。방법：35지 C57BL/6소서수궤분위5조，분별급여보통사료、불동제량적분찰패특（0.1%、0.25%、0.5%、0.75%）첨가보통사료위양4주후，응용역전록취합매련반응측정소서간장 SR-B1mRNA 표체。결과：불동제량분찰패특（0.1%、0.25%、0.5%、0.75%）간예후소서간장 SR-BImRNA 적표체분별위0.22±0.07、0.53±0.04、0.84±0.01、0.92±0.05，여대조조0.11±0.04비교，분찰패특명현증가소서간장 SR-BImRNA 적표체。결론：분찰패특능구상조간장 SR-B1mRNA 표체，촉진체내담고순역전운，리우동맥죽양경화방치。
[Abtract]ObjectiveAim This study is to observe effect of benzafibrate on The Scavenger receptor class B type I.(SR-BI) mRNA expression of liver. Methods 35 male C57BL/6 mice were randomly divided into four groups and treated with ordinary diet or plus different dosage (0.1%, 0.25%, 0.5%, 0.75% W/W) of bezafibrate for 4 weeks. Reverse transcription polymerase chain reaction (RT-PCR) was used to evaluate mRNA expression level of SR-BI. Results The SR-BImRNA expression of liver in mice treated with bezafibrate (0.1%, 0.25%, 0.5%, 0.75%) were upregulated by 0.22±0.07、0.53±0.04、0.84±0.01、0.92 ±0.05,compared to those in control group. Conclusions Bezafibrate upregulate the mRNA expression of SR-B1 to mediate the selective uptake of cholesteryl esters from HDL in the liver, so it will be in favor of the prevention of atherosclerosis.