中国耳鼻咽喉头颈外科
中國耳鼻嚥喉頭頸外科
중국이비인후두경외과
Chinese Archives of Otolaryngology-Head and Neck Surgery
2015年
10期
510-515
,共6页
苏娟%吉晓滨%谢景华%李雯
囌娟%吉曉濱%謝景華%李雯
소연%길효빈%사경화%리문
喉肿瘤%癌,鳞状细胞%半乳糖凝集素3%新生血管化,病理性%免疫组织化学
喉腫瘤%癌,鱗狀細胞%半乳糖凝集素3%新生血管化,病理性%免疫組織化學
후종류%암,린상세포%반유당응집소3%신생혈관화,병이성%면역조직화학
Laryngeal Neoplasms%Carcinoma,Squamous Cell%Galectin 3%Neovascularization,Pathologic%Immunohistochemistry
目的：探讨喉鳞状细胞癌（laryngeal squamous cell carcinoma，LSCC）中Endoglin（CD105）、β-半乳糖凝集素3（Galectin-3）蛋白的表达及与LSCC临床病理因素的关系。方法采用免疫组化SP法检测76例LSCC和25例喉正常黏膜中CD105、Galectin-3的表达。结果①LSCC中以CD105标记的微血管密度（microvessel density，MVD）为10.33±2.29，大于喉正常黏膜（1.20±1.04，t=18.732，P＜0.05）。CD105标记的MVD（CD105-MVD）的表达与LSCC的组织学分级、T分级、临床分期、淋巴结转移、是否复发、预后有关（P均＜0.05）。②Galectin-3蛋白表达的阳性率在LSCC为86.84%，大于喉正常黏膜36%（χ2=25.44， P＜0.05）。Galectin-3蛋白表达与LSCC的T分级、临床分期、淋巴结转移、是否复发、预后有关（P均＜0.05）。③CD105和Galectin-3蛋白表达呈正相关。④生存分析显示，CD105、Galectin-3、组织学分级、淋巴结转移、T分级、有无复发是患者预后独立的影响因素（P＜0.05）。结论 LSCC中CD105与Galectin-3蛋白的表达呈正相关，二者均促进LSCC的发生、恶性发展及不良预后，联合检测两者的表达有助于LSCC的诊断及预后判断。
目的：探討喉鱗狀細胞癌（laryngeal squamous cell carcinoma，LSCC）中Endoglin（CD105）、β-半乳糖凝集素3（Galectin-3）蛋白的錶達及與LSCC臨床病理因素的關繫。方法採用免疫組化SP法檢測76例LSCC和25例喉正常黏膜中CD105、Galectin-3的錶達。結果①LSCC中以CD105標記的微血管密度（microvessel density，MVD）為10.33±2.29，大于喉正常黏膜（1.20±1.04，t=18.732，P＜0.05）。CD105標記的MVD（CD105-MVD）的錶達與LSCC的組織學分級、T分級、臨床分期、淋巴結轉移、是否複髮、預後有關（P均＜0.05）。②Galectin-3蛋白錶達的暘性率在LSCC為86.84%，大于喉正常黏膜36%（χ2=25.44， P＜0.05）。Galectin-3蛋白錶達與LSCC的T分級、臨床分期、淋巴結轉移、是否複髮、預後有關（P均＜0.05）。③CD105和Galectin-3蛋白錶達呈正相關。④生存分析顯示，CD105、Galectin-3、組織學分級、淋巴結轉移、T分級、有無複髮是患者預後獨立的影響因素（P＜0.05）。結論 LSCC中CD105與Galectin-3蛋白的錶達呈正相關，二者均促進LSCC的髮生、噁性髮展及不良預後，聯閤檢測兩者的錶達有助于LSCC的診斷及預後判斷。
목적：탐토후린상세포암（laryngeal squamous cell carcinoma，LSCC）중Endoglin（CD105）、β-반유당응집소3（Galectin-3）단백적표체급여LSCC림상병리인소적관계。방법채용면역조화SP법검측76례LSCC화25례후정상점막중CD105、Galectin-3적표체。결과①LSCC중이CD105표기적미혈관밀도（microvessel density，MVD）위10.33±2.29，대우후정상점막（1.20±1.04，t=18.732，P＜0.05）。CD105표기적MVD（CD105-MVD）적표체여LSCC적조직학분급、T분급、림상분기、림파결전이、시부복발、예후유관（P균＜0.05）。②Galectin-3단백표체적양성솔재LSCC위86.84%，대우후정상점막36%（χ2=25.44， P＜0.05）。Galectin-3단백표체여LSCC적T분급、림상분기、림파결전이、시부복발、예후유관（P균＜0.05）。③CD105화Galectin-3단백표체정정상관。④생존분석현시，CD105、Galectin-3、조직학분급、림파결전이、T분급、유무복발시환자예후독립적영향인소（P＜0.05）。결론 LSCC중CD105여Galectin-3단백적표체정정상관，이자균촉진LSCC적발생、악성발전급불량예후，연합검측량자적표체유조우LSCC적진단급예후판단。
[ABSTRACT]OBJECTIVETo investigate the expressions of Endoglin (CD105) and Galectin-3 protein in laryngeal squamous cell carcinoma (LSCC) as well as the relationship between their expressions and the clinicopathological factors of LSCC.METHODSThe expressions of CD105 and Galectin-3 protein were detected in 76 samples of LSCC and 25 normal laryngeal tissues (NLT) by immunohistochemical staining (S-P).RESULTS 1.The mean of Microvessel density (MVD) value marked by CD105 in LSCC was 10.33±2.29, which was significantly higher than that in NLT (1.20±1.04) (P<0.05). The expression of MVD marked by CD105 (CD105-MVD) was correlated with histological grading, T stage, clinical stage, lymph node metastasis, recurrence and prognosis in LSCC (P<0.05). 2.The positive expression rate of Galectin-3 protein in LSCC was 86.84%, which was significantly higher than that in NLT (36%)(P<0.05). The expression of Galectin-3 was correlated with T stage,clinical stage, lymph node metastasis, recurrence and prognosis in LSCC (P<0.05). 3.There was a positive correlation between CD105 and Galectin-3 protein. 4.Survival analysis indicated that the expressions of CD105 and Galectin-3, histological grading, lymph node metastasis,T stage and recurrence were independent factors for tumor prognosis in LSCC (P<0.05). CONCLUSIONThe expressions of CD105 and Galectin-3 protein have a positive correlation in LSCC. They may play important roles in the tumorigenesis, malignant progression and poor prognosis of LSCC. Combined detection of them may be great value in diagnosis and predicting prognosis of LSCC.
