CAJ | 학술논문

目的通过去势建立绝经大鼠模型,观察绝经对大鼠血栓前状态的影响. 方法将32只9~10月龄的健康雌性SD大鼠随机分成4组,每组8只. 实验组手术去势,对照组行假手术,分别于术后1周或10周处死. 采用凝血酶比浊法检测各组大鼠血浆纤维蛋白原( FIB)含量;采用发射底物法检测抗凝血酶Ⅲ( ATⅢ)活性;采用ELISA双抗体夹心法检测纤溶酶原激活剂抑制剂(PAI-1)、D-二聚体(D-D)、血管性假血友病因子(vWF)含量;采用硝酸还原酶法检测NO含量;采用放射性核素放射免疫法检测血浆血栓素A2 ( TXA2 )、前列环素( PGI2 )含量. 结果在绝经早期,血浆FIB含量明显升高,血浆ATⅢ活性无明显变化;血浆PAI-1含量呈升高趋势;血浆vWF含量显著升高,同时血浆NO含量显著下降;随着绝经时间延长,到绝经晚期,与对照组相比,PAI-1含量显著增加,而血浆FIB含量有所回落,血浆ATⅢ活性显著升高;血浆vWF含量仍保持在较高水平,但血浆NO含量有所回升. 在绝经早期及绝经晚期,血浆TXA2 和PGI2 同步升高,D-D含量各组间均无明显变化. 结论在血栓形成的不同环节,绝经事件发挥了不同的影响,导致绝经早期血栓形成风险增加;随着绝经时间延长,各相关参数达成新的平衡,血栓风险回落.
목적 통과거세건립절경대서모형,관찰절경대대서혈전전상태적영향. 방법 장32지9~10월령적건강자성SD대서수궤분성4조,매조8지. 실험조수술거세,대조조행가수술,분별우술후1주혹10주처사. 채용응혈매비탁법검측각조대서혈장섬유단백원( FIB)함량;채용발사저물법검측항응혈매Ⅲ( ATⅢ)활성;채용ELISA쌍항체협심법검측섬용매원격활제억제제(PAI-1)、D-이취체(D-D)、혈관성가혈우병인자(vWF)함량;채용초산환원매법검측NO함량;채용방사성핵소방사면역법검측혈장혈전소A2 ( TXA2 )、전렬배소( PGI2 )함량. 결과 재절경조기,혈장FIB함량명현승고,혈장ATⅢ활성무명현변화;혈장PAI-1함량정승고추세;혈장vWF함량현저승고,동시혈장NO함량현저하강;수착절경시간연장,도절경만기,여대조조상비,PAI-1함량현저증가,이혈장FIB함량유소회락,혈장ATⅢ활성현저승고;혈장vWF함량잉보지재교고수평,단혈장NO함량유소회승. 재절경조기급절경만기,혈장TXA2 화PGI2 동보승고,D-D함량각조간균무명현변화. 결론 재혈전형성적불동배절,절경사건발휘료불동적영향,도치절경조기혈전형성풍험증가;수착절경시간연장,각상관삼수체성신적평형,혈전풍험회락.
Objective To observe the effects of menopause on prothrombotic profiles in ovariectomized rats .Methods Thirty two healthy female SD rats of 9 to 10 months were divided into 4 groups, the control shamed and the observation groups ovariectomized .Rats in the baseline group and the early menopause group were sacrificed one week later , and the control and late menopause group 10 weeks later.The prothrombotic profiles were detected including plasma FIB , ATⅢ activity, PAI-1 levels, D-D level, vWF levels and NO concentration, TXA2 and PGI2 levels.Results In early menopause, plasma FIB increased dramatically while ATⅢactivity remained lit-tle changed.PAI-1 demonstrated an increasing trend .vWF significantly increased but NO significantly decreased .In later menopausal stage, PAI-1 increased dramatically but FIB somewhat decreased .Plasma ATⅢactivity significantly increased and vWF still remained a high level.NO increased a little.In both early and later stage, TXA2 and PGI2 simultaneously increased while D -D showed little change between groups .Conclusion Menopause plays different roles in different aspects of thromoembolism , resulting in increased risk in early menopause due to prothrombotic state and decreased risk in later menopause when new balances between profiles were established .