医学研究杂志
醫學研究雜誌
의학연구잡지
Journal of Medical Research
2015年
10期
124-128
,共5页
胰腺癌%胰腺癌裸鼠皮下移植瘤%血管生成拟态%基质金属蛋白酶
胰腺癌%胰腺癌裸鼠皮下移植瘤%血管生成擬態%基質金屬蛋白酶
이선암%이선암라서피하이식류%혈관생성의태%기질금속단백매
Pancreatic cancer%Xenograft model of pancreatic cancer%Vasculogenic mimicry%Matrix metalloproteinase
目的 本实验通过建立胰腺癌SW-1990细胞株皮下移植瘤模型,探讨人参皂甙Rg3对胰腺癌皮下移植瘤血管生成拟态的影响. 方法 首先建立人胰腺癌细胞裸鼠模型,后给予不同药物浓度的人参皂甙Rg3(0、5、10、20mg/kg)处理裸鼠,观察人参皂甙Rg3对裸鼠移植瘤生长的影响,免疫组化-PAS双染观察人参皂甙Rg3对裸鼠胰腺癌皮下移植瘤血管生成拟态和血管内皮因子CD31的影响,并用荧光定量PCR和Western blot法分别检测MMP-2、MMP-9在mRNA和蛋白水平的表达. 结果人参皂甙Rg3能够抑制胰腺癌裸鼠皮下移植瘤的生长,其中20mg/kg人参皂甙Rg3组最明显;Western blot法和荧光定量PCR示用药组与空白组比较,MMP-2、MMP-9在蛋白和mRNA水平的表达下降,且差异有统计学意义(P<0.05);免疫组化-PAS双染后发现血管拟生(+)和CD31(+)数量均较空白组减少. 结论 通过体内实验笔者证实人参皂甙Rg3能够通过降低MMP-2、MMP-9的表达来抑制胰腺癌血管生成拟态的形成.
目的 本實驗通過建立胰腺癌SW-1990細胞株皮下移植瘤模型,探討人參皂甙Rg3對胰腺癌皮下移植瘤血管生成擬態的影響. 方法 首先建立人胰腺癌細胞裸鼠模型,後給予不同藥物濃度的人參皂甙Rg3(0、5、10、20mg/kg)處理裸鼠,觀察人參皂甙Rg3對裸鼠移植瘤生長的影響,免疫組化-PAS雙染觀察人參皂甙Rg3對裸鼠胰腺癌皮下移植瘤血管生成擬態和血管內皮因子CD31的影響,併用熒光定量PCR和Western blot法分彆檢測MMP-2、MMP-9在mRNA和蛋白水平的錶達. 結果人參皂甙Rg3能夠抑製胰腺癌裸鼠皮下移植瘤的生長,其中20mg/kg人參皂甙Rg3組最明顯;Western blot法和熒光定量PCR示用藥組與空白組比較,MMP-2、MMP-9在蛋白和mRNA水平的錶達下降,且差異有統計學意義(P<0.05);免疫組化-PAS雙染後髮現血管擬生(+)和CD31(+)數量均較空白組減少. 結論 通過體內實驗筆者證實人參皂甙Rg3能夠通過降低MMP-2、MMP-9的錶達來抑製胰腺癌血管生成擬態的形成.
목적 본실험통과건립이선암SW-1990세포주피하이식류모형,탐토인삼조대Rg3대이선암피하이식류혈관생성의태적영향. 방법 수선건립인이선암세포라서모형,후급여불동약물농도적인삼조대Rg3(0、5、10、20mg/kg)처리라서,관찰인삼조대Rg3대라서이식류생장적영향,면역조화-PAS쌍염관찰인삼조대Rg3대라서이선암피하이식류혈관생성의태화혈관내피인자CD31적영향,병용형광정량PCR화Western blot법분별검측MMP-2、MMP-9재mRNA화단백수평적표체. 결과인삼조대Rg3능구억제이선암라서피하이식류적생장,기중20mg/kg인삼조대Rg3조최명현;Western blot법화형광정량PCR시용약조여공백조비교,MMP-2、MMP-9재단백화mRNA수평적표체하강,차차이유통계학의의(P<0.05);면역조화-PAS쌍염후발현혈관의생(+)화CD31(+)수량균교공백조감소. 결론 통과체내실험필자증실인삼조대Rg3능구통과강저MMP-2、MMP-9적표체래억제이선암혈관생성의태적형성.
Objective To investigate the effects of ginsenosides Rg 3 on vasculogenic mimicry of pancreatic cancer xenograft through the establishment of pancreatic cancer cell line SW -1990 subcutaneous xenograft model .Methods After pancreatic cancer xenograft in nude mice model beening established , All the mice were randomly divided into 4 groups and treated intraperitoneally ( IP) with saline and various concentrations (5,10,20 mg/kg) of ginsenosides Rg3.To observe the effect of ginsenoside Rg3 on tumor growth.Immunohisto-chemical-PAS staining was used to detect the effects of ginsenosides Rg 3 on vasculogenic mimicry of pancreatic cancer xenograft .and mRNA and protein expression of MMP 2、MMP9 were respectively evaluated by FQ -PCR and Western blot .Results The ginsenosides Rg3 can inhibit the growth of the tumor xenografts in nude mice .The inhibitory effect is the most obvious the 20 mg/kg of ginsenosides Rg3 group.The expression of MMP-2, MMP-9 were down-regulated compare with the control group , and the difference was signifi-cant;the Immunohistochemical -PAS staining showed the number of vasculogenic mimicry (+) and CD31 (+) were less than that in the control group .Conlusion Our results demonstrate that pancreatic vascular mimicry formation can be suppressed by Ginsenoside Rg 3 though reducing the expression of MMP -2, MMP-9 in our vivo experiments ,
