长治医学院学报
長治醫學院學報
장치의학원학보
Journal of Changzhi Medical College
2015年
5期
333-336
,共4页
微柱%表皮细胞%内皮细胞%成纤维细胞%细胞核形态
微柱%錶皮細胞%內皮細胞%成纖維細胞%細胞覈形態
미주%표피세포%내피세포%성섬유세포%세포핵형태
micropillar%epithelial cell%fibroblast%endothelial cell%cell nucleus shape
目的：：探讨微柱对3种细胞细胞核形态的影响。方法：在高度10μm,直径18μm,间距15μm 的 PDMS 微柱上分别培养3种细胞(表皮细胞、内皮细胞和成纤维细胞),24 h 后对其细胞核形态参数包括平均面积、周长、纵横比以及圆度值进行测量,并与其在平面上的相应值进行比较分析。结果：微柱上表皮细胞和内皮细胞的细胞核变形明显,核平均面积较平面上小很多,出现了弯月形和哑铃形；而成纤维细胞核则变形较小,仍为椭圆形,只是纵横比增大。结论：本实验采用的微柱尺度会使得3种细胞有不同程度的细胞核变形。
目的：：探討微柱對3種細胞細胞覈形態的影響。方法：在高度10μm,直徑18μm,間距15μm 的 PDMS 微柱上分彆培養3種細胞(錶皮細胞、內皮細胞和成纖維細胞),24 h 後對其細胞覈形態參數包括平均麵積、週長、縱橫比以及圓度值進行測量,併與其在平麵上的相應值進行比較分析。結果：微柱上錶皮細胞和內皮細胞的細胞覈變形明顯,覈平均麵積較平麵上小很多,齣現瞭彎月形和啞鈴形；而成纖維細胞覈則變形較小,仍為橢圓形,隻是縱橫比增大。結論：本實驗採用的微柱呎度會使得3種細胞有不同程度的細胞覈變形。
목적：：탐토미주대3충세포세포핵형태적영향。방법：재고도10μm,직경18μm,간거15μm 적 PDMS 미주상분별배양3충세포(표피세포、내피세포화성섬유세포),24 h 후대기세포핵형태삼수포괄평균면적、주장、종횡비이급원도치진행측량,병여기재평면상적상응치진행비교분석。결과：미주상표피세포화내피세포적세포핵변형명현,핵평균면적교평면상소흔다,출현료만월형화아령형；이성섬유세포핵칙변형교소,잉위타원형,지시종횡비증대。결론：본실험채용적미주척도회사득3충세포유불동정도적세포핵변형。
Objective:Effects of micropillar on cell nucleus shapes of three different kinds of cells was discussed.Methods:First, these three different kinds of cells(epithelial cell,fibroblast and endothelial cell)were cultured on an array of PDMS micropillars which were 10 μm in height,18 μm in diameter and 15 μm in edge to edge spacing.Then,a quantitative and comparative analysis of the cell nucleus shapes was given by measuring the average area,aspect ratio and their circularity of the cells both on the micropillars and on the flat substrate.Results:Distinct nuclear deformation such as crescent,and dumbbell was observed of epithelial cells and endothelial cells on the micropillar substrate and their nuclear average areas were much smaller than that of the the cells on the flat substrate,when nucleus shape of fibroblasts was still in oval but just with a larger aspect ratio.Conclusion:Different degree of nuclear deformation would be induced by the micropillars that we used in this paper.
