山西医科大学学报
山西醫科大學學報
산서의과대학학보
Journal of Shanxi Medical University
2015年
10期
959-961
,共3页
右美托咪定%心肌%缺血再灌注损伤%血管内皮生长因子
右美託咪定%心肌%缺血再灌註損傷%血管內皮生長因子
우미탁미정%심기%결혈재관주손상%혈관내피생장인자
dexmedetomidine%myocardium%ischemia-reperfusion injury%vascular endothelial growth factor( VEGF)
目的 观察右美托咪定预处理对心肌缺血再灌注损伤( MIRI)大鼠VEGF表达的影响,探讨其对缺血再灌注心肌的保护作用. 方法 将40 只健康雄性SD大鼠,随机分为4 组:假手术组(A),MIRI组(B),MIRI+右美托咪定10 μg/kg组( C) ,MIRI+右美托咪定30 μg/kg组( D). 采用结扎冠脉左前降支的方法制作心肌缺血再灌注模型,免疫组化法测定各组大鼠心肌组织中VEGF的表达. 结果 与A组相比,B组、C组VEGF表达均减少(P<0. 05);与B组相比,C组、D组VEGF表达均增加(P<0. 05);与C组相比,D组VEGF表达增加(P<0. 05). 结论 右美托咪定通过预处理增加心肌缺血再灌注损伤大鼠心肌VEGF的表达保护心肌,且其作用与剂量有关.
目的 觀察右美託咪定預處理對心肌缺血再灌註損傷( MIRI)大鼠VEGF錶達的影響,探討其對缺血再灌註心肌的保護作用. 方法 將40 隻健康雄性SD大鼠,隨機分為4 組:假手術組(A),MIRI組(B),MIRI+右美託咪定10 μg/kg組( C) ,MIRI+右美託咪定30 μg/kg組( D). 採用結扎冠脈左前降支的方法製作心肌缺血再灌註模型,免疫組化法測定各組大鼠心肌組織中VEGF的錶達. 結果 與A組相比,B組、C組VEGF錶達均減少(P<0. 05);與B組相比,C組、D組VEGF錶達均增加(P<0. 05);與C組相比,D組VEGF錶達增加(P<0. 05). 結論 右美託咪定通過預處理增加心肌缺血再灌註損傷大鼠心肌VEGF的錶達保護心肌,且其作用與劑量有關.
목적 관찰우미탁미정예처리대심기결혈재관주손상( MIRI)대서VEGF표체적영향,탐토기대결혈재관주심기적보호작용. 방법 장40 지건강웅성SD대서,수궤분위4 조:가수술조(A),MIRI조(B),MIRI+우미탁미정10 μg/kg조( C) ,MIRI+우미탁미정30 μg/kg조( D). 채용결찰관맥좌전강지적방법제작심기결혈재관주모형,면역조화법측정각조대서심기조직중VEGF적표체. 결과 여A조상비,B조、C조VEGF표체균감소(P<0. 05);여B조상비,C조、D조VEGF표체균증가(P<0. 05);여C조상비,D조VEGF표체증가(P<0. 05). 결론 우미탁미정통과예처리증가심기결혈재관주손상대서심기VEGF적표체보호심기,차기작용여제량유관.
Objective To explore the effect of dexmedetomidine on the expression of vascular endothelial growth factor( VEGF) in my-ocardium of myocardial ischemia-reperfusion injury rats. Methods Forty healthy male SD rats were randomly divided into 4 groups:sham group( A) , MIRI group( B) , MIRI+Dex 10 μg/kg group( C) , MIRI+Dex 30 μg/kg group( D) . The left anterior descending coronary artery was ligated for establishing the rat myocardial ischemia-reperfusion injury model in vivo. Immunohistochemistry was used to analyze the expression of VEGF. Results Compared with group A,the expression of VEGF was reduced in group B and group C(P<0. 05). Compared with group B, the expression of VEGF was increased in group C and group D(P<0. 05). The expression in group D was significantly higher than in group C(P<0. 05). Conclusion Preconditioning of dexmedetomidine can have protective effects on the myocardium by up-regulating the expression of VEGF.
