CAJ | 학술논문

目的探讨大黄素对人胶质瘤SHG44细胞增殖、体外迁移和侵袭能力的影响. 方法实验组大黄素浓度分别为10,20,40 μmol/L和80 μmol/L,并设对照组(未经大黄素处理). 用噻唑蓝( MTT)法观察大黄素处理12,24,36 h和48 h后SHG44细胞的增殖情况;流式细胞仪分析细胞周期变化;通过Transwell小室迁移和侵袭实验,观察大黄素对SHG44细胞体外迁移和侵袭能力的影响. 结果与对照组相比,大黄素处理胶质瘤SHG44细胞后,MTT法及流式细胞仪分析大黄素能浓度依赖性抑制SHG44细胞增殖(P<0. 05);对于细胞周期的影响主要表现为引起细胞发生G0/G1 期阻滞;Transwell小室迁移和侵袭实验表明大黄素处理后的SHG44细胞迁移和侵袭能力较对照组明显降低(P<0. 05). 结论大黄素能通过阻滞细胞周期于G0/G1 期而抑制胶质瘤SHG44细胞的增殖,降低细胞的体外迁移及侵袭能力.
목적 탐토대황소대인효질류SHG44세포증식、체외천이화침습능력적영향. 방법 실험조대황소농도분별위10,20,40 μmol/L화80 μmol/L,병설대조조(미경대황소처리). 용새서람( MTT)법관찰대황소처리12,24,36 h화48 h후SHG44세포적증식정황;류식세포의분석세포주기변화;통과Transwell소실천이화침습실험,관찰대황소대SHG44세포체외천이화침습능력적영향. 결과 여대조조상비,대황소처리효질류SHG44세포후,MTT법급류식세포의분석대황소능농도의뢰성억제SHG44세포증식(P<0. 05);대우세포주기적영향주요표현위인기세포발생G0/G1 기조체;Transwell소실천이화침습실험표명대황소처리후적SHG44세포천이화침습능력교대조조명현강저(P<0. 05). 결론 대황소능통과조체세포주기우G0/G1 기이억제효질류SHG44세포적증식,강저세포적체외천이급침습능력.
Objective To investigate the effects of emodin on proliferation,migration and invasion in vitro of human glioma SHG44 cells. Methods SHG44 cells were treated with different concentrations of emodin(0,10,20,40 and 80μmol/L). The cell prolifera-tion at 12,24,36, 48 h and cell cycle were detected by MTT assay and flow cytometry respectively. The migration ability and invasion ability were assessed by Transwell assay. Results The emodin significantly inhibited the proliferation of SHG44 cells in a dose-de-pendent and time-dependent manner. Flow cytometry indicated that cell cycle was arrested in the G0/G1 phase by emodin. The migra-tion ability and invasion ability was decreased after treated with emodin(P<0. 05). Conclusion Emodin can inhibit the proliferation of glioma SHG44 cells by cell cycle arrest in the G0/G1 phase, thus decrease the migration ability and invasion ability in vitro.