实用医院临床杂志
實用醫院臨床雜誌
실용의원림상잡지
Practical Journal of Clinical Medicine
2015年
6期
31-33,34
,共4页
杨永长%肖代雯%喻华%姜伟%陈亮%胡洪华%周薇%黄文芳
楊永長%肖代雯%喻華%薑偉%陳亮%鬍洪華%週薇%黃文芳
양영장%초대문%유화%강위%진량%호홍화%주미%황문방
耐甲氧西林表皮葡萄球菌%psm-mec%耐药谱
耐甲氧西林錶皮葡萄毬菌%psm-mec%耐藥譜
내갑양서림표피포도구균%psm-mec%내약보
Methicillin resistant Staphylococcus epidermidis%Psm-mec%Antimicrobial resistance profile
目的:比较携带与未携带psm-mec基因耐甲氧西林表皮葡萄球菌( MRSE)的耐药谱,为治疗MRSE引起的感染提供理论依据。方法收集临床分离并经过全自动微生物奠定系统准确鉴定的表皮葡萄球菌( Staphylococcus epidermidis,S. epidermidis)165株,通过PCR扩增esp和mecA基因,准确鉴定和区分甲氧西林敏感表皮葡萄球菌( MSSE)和MRSE。扩增psm-mec,fudoh和p221片段确认携带psm-mec基因的MRSE菌株,比较分析携带与未携带psm-mec 基因MRSE的耐药谱。结果83.64%的临床分离S.epidermidis为MRSE,其中29株携带psm-mec基因,携带率为17.58%,且仅分布于MRSE中。临床分离MRSE易对苯唑西林、青霉素、红霉素、复方新诺明和克林霉素耐药,未发现对利奈唑胺、呋喃妥因、普丁/达福、万古霉素和替加环素耐药菌株。携带psm-mec基因MRSE对环丙沙星、庆大霉素、利福平和复方新诺明的耐药率明显高于未携带psm-mec基因MRSE。结论携带psm-mec基因MRSE易对苯唑西林、青霉素、红霉素、克林霉素、环丙沙星、庆大霉素、利福平和复方新诺明耐药。
目的:比較攜帶與未攜帶psm-mec基因耐甲氧西林錶皮葡萄毬菌( MRSE)的耐藥譜,為治療MRSE引起的感染提供理論依據。方法收集臨床分離併經過全自動微生物奠定繫統準確鑒定的錶皮葡萄毬菌( Staphylococcus epidermidis,S. epidermidis)165株,通過PCR擴增esp和mecA基因,準確鑒定和區分甲氧西林敏感錶皮葡萄毬菌( MSSE)和MRSE。擴增psm-mec,fudoh和p221片段確認攜帶psm-mec基因的MRSE菌株,比較分析攜帶與未攜帶psm-mec 基因MRSE的耐藥譜。結果83.64%的臨床分離S.epidermidis為MRSE,其中29株攜帶psm-mec基因,攜帶率為17.58%,且僅分佈于MRSE中。臨床分離MRSE易對苯唑西林、青黴素、紅黴素、複方新諾明和剋林黴素耐藥,未髮現對利奈唑胺、呋喃妥因、普丁/達福、萬古黴素和替加環素耐藥菌株。攜帶psm-mec基因MRSE對環丙沙星、慶大黴素、利福平和複方新諾明的耐藥率明顯高于未攜帶psm-mec基因MRSE。結論攜帶psm-mec基因MRSE易對苯唑西林、青黴素、紅黴素、剋林黴素、環丙沙星、慶大黴素、利福平和複方新諾明耐藥。
목적:비교휴대여미휴대psm-mec기인내갑양서림표피포도구균( MRSE)적내약보,위치료MRSE인기적감염제공이론의거。방법수집림상분리병경과전자동미생물전정계통준학감정적표피포도구균( Staphylococcus epidermidis,S. epidermidis)165주,통과PCR확증esp화mecA기인,준학감정화구분갑양서림민감표피포도구균( MSSE)화MRSE。확증psm-mec,fudoh화p221편단학인휴대psm-mec기인적MRSE균주,비교분석휴대여미휴대psm-mec 기인MRSE적내약보。결과83.64%적림상분리S.epidermidis위MRSE,기중29주휴대psm-mec기인,휴대솔위17.58%,차부분포우MRSE중。림상분리MRSE역대분서서림、청매소、홍매소、복방신낙명화극림매소내약,미발현대리내서알、부남타인、보정/체복、만고매소화체가배소내약균주。휴대psm-mec기인MRSE대배병사성、경대매소、리복평화복방신낙명적내약솔명현고우미휴대psm-mec기인MRSE。결론휴대psm-mec기인MRSE역대분서서림、청매소、홍매소、극림매소、배병사성、경대매소、리복평화복방신낙명내약。
Objective To compare the antimicrobial resistance profiles between MRSE with and without psm-mec gene in or-der to provide foundation for treatment of MRSE infection disease.Methods One hundred and sixty five strains of S.epidermidis iden-tified accurately by full automation microbiological identification system were collected.PCR was used to amplify esp and mecA gene to differentiate methicillin-resistant S.epidermidis ( MRSE) and methicillin-sensitive S.epidermidis ( MSSE) .Strains with psm-mec gene were validated by amplification of psm-mec,fudoh and p221 fragments,and antimicrobial resistance profile of MRSE with or without psm-mec gene was analyzed.Results MRSE accounted for 83.64%of clinical isolated S.epidermidis,and 29 strains were positive for psm-mec gene with a rate of 17.58%,which were distributed only in MRSE.Clinical isolates of MRSE were usually resistant to oxacil-lin,penicillin,erythromycin,compound sulfamethoxazole and clindamycin,but no strains resistant to linezolid,furantoin,quinupristin/dalfopristin,vancomycin and tigecycline were found.The rate of MRSE with psm-mec gene resistant to ciprofloxacin, gentamicin, ri-fampin and compound sulfamethoxazole was higher than that of MRSE without psm-mec.Conclusion MRSE with psm-mec gene are more likely resistant to oxacillin,penicillin,erythromycin,clindamycin,ciprofloxacin,gentamicin,rifampin and sulfamethoxazole-trime-thoprim.