CAJ | 학술논문

目的调查云南傣族和广西侗族无关个体18个STR基因座(D18S51、D21S11、D3S1358、FGA、D8S1179、vWA、CSF1PO、D16S539、D7S820、D13S317、D5S818、D2S1338、D19S433、D12S391、TPOX、TH01、Penta E和D6S1043)的遗传多态性并研究其法医学应用价值. 方法采用DNA TyperTM 19试剂盒对100名云南傣族和70名广西侗族的无关个体血样进行复合扩增,用遗传分析仪3130XL对扩增产物检测,用GeneMapper ID v3. 2软件进行基因分型,并计算群体遗传学参数. 结果在100名云南傣族和70名广西侗族的无关个体中,云南傣族共发现185种等位基因,广西侗族共发现162种等位基因. 傣族单个等位基因频率分布在0. 005-0. 600、侗族在0. 007-0. 493;傣族与侗族累计个人识别能力均大于99. 999 999 999 999 999 999 99%.结论这18个 STR基因座在云南傣族和广西侗族地区具有高度多态性,可满足对这两个群体的个体识别和亲权鉴定.
목적 조사운남태족화엄서동족무관개체18개STR기인좌(D18S51、D21S11、D3S1358、FGA、D8S1179、vWA、CSF1PO、D16S539、D7S820、D13S317、D5S818、D2S1338、D19S433、D12S391、TPOX、TH01、Penta E화D6S1043)적유전다태성병연구기법의학응용개치. 방법 채용DNA TyperTM 19시제합대100명운남태족화70명엄서동족적무관개체혈양진행복합확증,용유전분석의3130XL대확증산물검측,용GeneMapper ID v3. 2연건진행기인분형,병계산군체유전학삼수. 결과 재100명운남태족화70명엄서동족적무관개체중,운남태족공발현185충등위기인,엄서동족공발현162충등위기인. 태족단개등위기인빈솔분포재0. 005-0. 600、동족재0. 007-0. 493;태족여동족루계개인식별능력균대우99. 999 999 999 999 999 999 99%.결론 저18개 STR기인좌재운남태족화엄서동족지구구유고도다태성,가만족대저량개군체적개체식별화친권감정.
Objective To investigate the genetic polymorphisms of 18 STR loci(D18S51, D21S11, D3S1358, FGA, D8S1179, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818, D2S1338, D19S433, D12S391, TPOX, TH01, Penta E and D6S1043) in unre-lated Dai individuals in Yunnan and Dong individuals in Guangxi and to explore its application value in forensic practice. Methods Blood samples from unrelated 100 Dai and 70 Dong individuals were amplified using DNA TyperTM 19 kit. The amplified products were detected using 3130XL Genetic Analyzer, and the genotyping was done using GeneMapper ID v3. 2, and the population genetics param-eters were calculated. Results Of 100 Dai and 70 Dong unrelated individuals, 185 alleles were detected in Dai population and 162 alleles were detected in Dong population. The allele frequency was 0. 005-0. 600 for Dai population and 0. 007-0. 493 for Dong pop-ulation. The TDP of both Dai and Dong population was more than 99. 999 999 999 999 999 999 99%. Conclusion The 18 STR loci in Dai population of Yunnan and Dong population of Guangxi have high genetic polymorphisms, and can be genetic markers for popula-tion individual identification and paternity testing.