CAJ | 학술논문

目的检测狼毒大戟中活性成分没食子酸乙酯Ethyl gallate( EG)对乳腺癌细胞生长的影响. 方法以两种乳腺癌细胞MDA-MB-231、MCF-7为研究对象,采用MTT法检测不同浓度EG作用乳腺癌细胞24 h、48 h、72 h后对细胞生长的影响;采用流式细胞术分析EG作用后乳腺癌细胞的凋亡作用;采用免疫荧光染色法观察细胞的形态学变化;采用western blot检测凋亡相关蛋白Bcl-2、Bax蛋白的表达.结果 EG对人乳腺癌MDA-MB-231、MCF-7细胞增殖具有明显抑制作用,呈浓度、时间依赖性;可明显诱导MDA-MB-231细胞凋亡,凋亡率高达(P<0.05),荧光显微镜下可见凋亡的典型形态学变化;与空白对照组相比,EG处理组可使凋亡相关蛋白Bcl-2/Bax表达明显下降( P<0.05). 结论体外实验中,EG可抑制乳腺癌细胞的生长,其可能的机制是促进细胞凋亡,抑制凋亡相关蛋白Bcl-2/Bax表达有关.
목적 검측랑독대극중활성성분몰식자산을지Ethyl gallate( EG)대유선암세포생장적영향. 방법 이량충유선암세포MDA-MB-231、MCF-7위연구대상,채용MTT법검측불동농도EG작용유선암세포24 h、48 h、72 h후대세포생장적영향;채용류식세포술분석EG작용후유선암세포적조망작용;채용면역형광염색법관찰세포적형태학변화;채용western blot검측조망상관단백Bcl-2、Bax단백적표체.결과 EG대인유선암MDA-MB-231、MCF-7세포증식구유명현억제작용,정농도、시간의뢰성;가명현유도MDA-MB-231세포조망,조망솔고체(P<0.05),형광현미경하가견조망적전형형태학변화;여공백대조조상비,EG처리조가사조망상관단백Bcl-2/Bax표체명현하강( P<0.05). 결론 체외실험중,EG가억제유선암세포적생장,기가능적궤제시촉진세포조망,억제조망상관단백Bcl-2/Bax표체유관.
Objective To study on the effects of Ethyl gallate ( EG) from Euphorbia fischeriana Steud extracts on growth of breast cancer cell.Methods MTT was performed to determine the proliferation inhibiting effect of EG at different concentration and time.Apoptosis were examined by flow cytometry, and the cellular morphological changes was observed by immunofluorescence staining.Apoptosis related proteins the Bcl-2, Bax protein expression was detected by western blot. Results EG obviously suppressed breast cancer cells proliferation in dose-and time-dependent manners and induce apoptosis, fluorescence microscopy shows the typical morphological changes of apoptosis.The expression of apoptosis related proteins the Bcl-2/Bax obviously decreased after EG treatment compared with the blank control group (P<0.05).Conclusions EG suppressed cells proliferation.Its possible mechanism is to induce apoptosis by altering Bcl-2/Bax ratio.