CAJ | 학술논문

目的建立可测定螺内酯片剂中螺内酯含量的毛细管电泳法. 方法采用BECKMAN毛细管电泳仪;未涂层毛细管(75 μm × 57 cm,有效长度10. 2 cm);二极管阵列检测器( DAD);以含0. 004 mmol/L的十六烷基三甲基溴化铵( CTAB)和20%乙腈的pH6. 5磷酸盐缓冲液为背景电解质溶液,浓度为0. 020 mol/L;分离电压20 kV;检测波长244 nm. 结果螺内酯在1. 62-119. 9 μg/ml浓度范围内线性关系良好,线性方程为Y=877. 7X+4 238 (r2 = 0. 991 6);回收率91. 2%-106. 6%;精密度、稳定性、重复性均较好. 结论毛细管电泳法测定螺内酯片中螺内酯含量,方法简便、快速、专属性强,可用于药物制剂中螺内酯含量的测定.
목적 건립가측정라내지편제중라내지함량적모세관전영법. 방법 채용BECKMAN모세관전영의;미도층모세관(75 μm × 57 cm,유효장도10. 2 cm);이겁관진렬검측기( DAD);이함0. 004 mmol/L적십륙완기삼갑기추화안( CTAB)화20%을정적pH6. 5린산염완충액위배경전해질용액,농도위0. 020 mol/L;분리전압20 kV;검측파장244 nm. 결과 라내지재1. 62-119. 9 μg/ml농도범위내선성관계량호,선성방정위Y=877. 7X+4 238 (r2 = 0. 991 6);회수솔91. 2%-106. 6%;정밀도、은정성、중복성균교호. 결론 모세관전영법측정라내지편중라내지함량,방법간편、쾌속、전속성강,가용우약물제제중라내지함량적측정.
Objective To establish a method for determining the content of spironolactone in spironolactone tablet by capillary electro-phoresis. Methods Capillary electrophoresis apparatus with diode array detector was adopted. The uncoated fused silica capillary (75μm × 57 cm, effective length of 10. 2 cm) was available. The pH 6. 5 phosphate buffer solution containing 20% acetonitrile and 0. 004 mmol/L cetyl trimethyl ammonium bromide( CTAB) were chosen as the background electrolyte, and the concentration of background e-lectrolyte was 0. 020 mol/L. The analysis voltage was set at 20 kV and the detection wavelength was set at 244 nm. Results There was a good linear relationship between the peak area and the concentration of spironolactone in the range of 1. 62-119. 9 μg/ml, and the linear regression equation was Y=877. 7X+4 238 (r2 =0. 991 6). The recovery rate was 91. 2%-106. 6%. The precision, sta-bility, repeatability of the method were good. Conclusion The developed method is simple, rapid and specific, which can be used for the determination of spironolactone in pharmaceutical preparation.