中国药师
中國藥師
중국약사
China Pharmacist
2015年
11期
1978-1980
,共3页
程道海%王捷%陆华%黄振光
程道海%王捷%陸華%黃振光
정도해%왕첩%륙화%황진광
舒血灵胶囊%三七皂苷R1%人参皂苷Rg1%人参皂苷Rb1%高效液相色谱法
舒血靈膠囊%三七皂苷R1%人參皂苷Rg1%人參皂苷Rb1%高效液相色譜法
서혈령효낭%삼칠조감R1%인삼조감Rg1%인삼조감Rb1%고효액상색보법
Shuxuening capsules%Notoginsenoside R1%Ginsenoside Rg1%Ginsenoside Rb1%HPLC
目的::建立舒血灵胶囊中三七皂苷R1、人参皂苷Rg1及人参皂苷Rb1的含量测定方法。方法:采用Hypersil ODS-2 C18(250 mm ×4.6 mm,5μm)色谱柱;以乙腈(A)-水(B)为流动相进行梯度洗脱,流动相梯度为:0~8 min,20%A→20%A,8~40 min,20%A→30%A,40~60 min,30%A→45%A;流速:1.0 ml·min-1;柱温:25℃;检测波长:203 nm;进样量:20μl。结果:三七皂苷R1在浓度0.05~0.50 mg·ml-1范围内,人参皂苷Rg1和Rb1在浓度0.20~2.00 mg·ml-1范围内,与峰面积呈较好的线性关系(r =0.9999);三种成分的平均加样回收率分别为98.79%、98.42%、98.89%,RSD 分别为0.85%、0.97%、0.74%(n=6);日内精密度分别为0.49%、0.20%和0.39%;日间精密度分别为0.75%、0.56%和0.51%;稳定性、重复性试验的RSD<1%。结论:本试验建立的测定方法简便、准确性高、重复性好,可用于该制剂的含量测定。
目的::建立舒血靈膠囊中三七皂苷R1、人參皂苷Rg1及人參皂苷Rb1的含量測定方法。方法:採用Hypersil ODS-2 C18(250 mm ×4.6 mm,5μm)色譜柱;以乙腈(A)-水(B)為流動相進行梯度洗脫,流動相梯度為:0~8 min,20%A→20%A,8~40 min,20%A→30%A,40~60 min,30%A→45%A;流速:1.0 ml·min-1;柱溫:25℃;檢測波長:203 nm;進樣量:20μl。結果:三七皂苷R1在濃度0.05~0.50 mg·ml-1範圍內,人參皂苷Rg1和Rb1在濃度0.20~2.00 mg·ml-1範圍內,與峰麵積呈較好的線性關繫(r =0.9999);三種成分的平均加樣迴收率分彆為98.79%、98.42%、98.89%,RSD 分彆為0.85%、0.97%、0.74%(n=6);日內精密度分彆為0.49%、0.20%和0.39%;日間精密度分彆為0.75%、0.56%和0.51%;穩定性、重複性試驗的RSD<1%。結論:本試驗建立的測定方法簡便、準確性高、重複性好,可用于該製劑的含量測定。
목적::건립서혈령효낭중삼칠조감R1、인삼조감Rg1급인삼조감Rb1적함량측정방법。방법:채용Hypersil ODS-2 C18(250 mm ×4.6 mm,5μm)색보주;이을정(A)-수(B)위류동상진행제도세탈,류동상제도위:0~8 min,20%A→20%A,8~40 min,20%A→30%A,40~60 min,30%A→45%A;류속:1.0 ml·min-1;주온:25℃;검측파장:203 nm;진양량:20μl。결과:삼칠조감R1재농도0.05~0.50 mg·ml-1범위내,인삼조감Rg1화Rb1재농도0.20~2.00 mg·ml-1범위내,여봉면적정교호적선성관계(r =0.9999);삼충성분적평균가양회수솔분별위98.79%、98.42%、98.89%,RSD 분별위0.85%、0.97%、0.74%(n=6);일내정밀도분별위0.49%、0.20%화0.39%;일간정밀도분별위0.75%、0.56%화0.51%;은정성、중복성시험적RSD<1%。결론:본시험건립적측정방법간편、준학성고、중복성호,가용우해제제적함량측정。
Objective:To determine the content of notoginsenoside R1 , ginsenoside Rg1 and Rb1 in Shuxuening capsules. Meth-ods:The three constituents were determined on a Hypersil ODS-2 C18 column (250 mm × 4. 6 mm, 5 μm) with gradient elution using acetonitrile (A) -aqueous solution (B) (0-8 min, 20%A→20%A, 8-40 min, 20%A→30%A, 40-60 min, 30%A→45%A) at the detection wavelength of 203 nm with a flow rate of 1. 0 ml·min-1 . The column temperature was 25℃ and the injection volume was 20μl. Results:The calibration curve showed good linearity within the concentration range of 0. 05-0. 50 mg·ml-1 for notogisenoside R1 , and 0.20-2.00 mg·ml-1 for ginsenoside Rg1 and Rb1(r =0.999 9). The average relative recovery was 98.79%, 98.42% and 98. 89% for each constituent(RSD=0. 85%, 0. 97% and 0. 74%, respectively, n=6). The intra-day RSD was 0. 49%, 0. 20% and 0. 39%, and the inter-day RSD was 0. 75%, 0. 56% and 0. 51%, respectively. The RSDs of stability test and repeatability test were less than 1%. Conclusion:The method is simple with good accuracy and repeatability, which can be used for the determination of no-toginsenoside R1 , ginsenoside Rg1 and Rb1 in Shuxuening capsules.