中国药师
中國藥師
중국약사
China Pharmacist
2015年
11期
2008-2010
,共3页
柱前衍生化%夜宁颗粒%二苯乙烯苷%反相高效液相色谱%丹酰氯
柱前衍生化%夜寧顆粒%二苯乙烯苷%反相高效液相色譜%丹酰氯
주전연생화%야저과립%이분을희감%반상고효액상색보%단선록
Pre-column derivation%Yening granules%Stilbene glycosides%RP-HPLC%DNS-Cl
目的：：建立柱前衍生化RP-HPLC法测定夜宁颗粒中二苯乙烯苷含量的方法。方法：采用丹酰氯作为衍生化试剂,色谱柱为Wondasil-C8色谱柱(250 mm ×4.6 mm,5μm),以(30 mM的乙酸铵用磷酸调节pH至3.5)-乙腈(78∶22)为流动相,柱温为30℃,流速为1.0 ml·min-1,荧光检测器检测(激发波长为370 nm,发射波长为560 nm),进样量为20μl。结果：制剂中其他成分无干扰,二苯乙烯苷在0.071~27.280 mg·ml-1范围内线性关系良好,r=0.9999,平均回收率为99.04%,RSD为0.1%(n=9)。结论：所用方法简便快捷,灵敏度高,准确性好,可作为夜宁颗粒质量控制方法的依据。
目的：：建立柱前衍生化RP-HPLC法測定夜寧顆粒中二苯乙烯苷含量的方法。方法：採用丹酰氯作為衍生化試劑,色譜柱為Wondasil-C8色譜柱(250 mm ×4.6 mm,5μm),以(30 mM的乙痠銨用燐痠調節pH至3.5)-乙腈(78∶22)為流動相,柱溫為30℃,流速為1.0 ml·min-1,熒光檢測器檢測(激髮波長為370 nm,髮射波長為560 nm),進樣量為20μl。結果：製劑中其他成分無榦擾,二苯乙烯苷在0.071~27.280 mg·ml-1範圍內線性關繫良好,r=0.9999,平均迴收率為99.04%,RSD為0.1%(n=9)。結論：所用方法簡便快捷,靈敏度高,準確性好,可作為夜寧顆粒質量控製方法的依據。
목적：：건립주전연생화RP-HPLC법측정야저과립중이분을희감함량적방법。방법：채용단선록작위연생화시제,색보주위Wondasil-C8색보주(250 mm ×4.6 mm,5μm),이(30 mM적을산안용린산조절pH지3.5)-을정(78∶22)위류동상,주온위30℃,류속위1.0 ml·min-1,형광검측기검측(격발파장위370 nm,발사파장위560 nm),진양량위20μl。결과：제제중기타성분무간우,이분을희감재0.071~27.280 mg·ml-1범위내선성관계량호,r=0.9999,평균회수솔위99.04%,RSD위0.1%(n=9)。결론：소용방법간편쾌첩,령민도고,준학성호,가작위야저과립질량공제방법적의거。
Objective:To establish a RP-HPLC method with pre-column derivation for determining the content of stilbene glyco-sides in Yening granules. Methods:DNS-Cl was used as the derivation reagent, a Wondasil-C8 column (250 mm × 4. 6 mm, 5 μm) was used as the stationary phase and 30mM ammonium phosphatere gulation (pH 3. 5)-acetonitrile (78∶22) was applied as the mobile phase, a fluorescence detector (λex=370 nm,λem=560 nm) was used, the flow rate was 1. 0 ml·ml-1 , the column temperature was 30℃ and the injection volume was 20 μl. Results: There was no interference from the other substances in the preparation. The linear range of the two stilbene glycosides was 0.071-27.280 mg·ml-1(r=0.999 9). The average recovery was 99.04%(RSD=0. 1%, n= 9). Conclusion:The method is simple, quick, sensitive and accurate, which can be used for the quality control of Ye-ning granules.
