CAJ | 학술논문

血管支架植入后血栓及再狭窄依然是临床上的主要问题. 通过材料表面仿生微环境的构建改善支架抗凝性能并加速血管内皮修复是解决上述问题的主要手段. 研究表明,VEGF和SDF-1α均可有效介导血管内皮细胞的增殖与迁移.为比较两类因子在内皮化过程中的功能差异,选择在材料表面构建纤连蛋白/肝素功能层,并分别引入VEGF与 SDF-1α两种因子,通过XPS和水接触角检测研究修饰表面的理化性质. 体外动态释放实验结果显示,修饰层具有良好的控制因子释放的能力. 体外细胞相容性评价结果显示,VEGF和SDF-1α均具有刺激内皮细胞增殖和迁移的功能,但VEGF表现出更强烈的调节内皮细胞行为的功能.
혈관지가식입후혈전급재협착의연시림상상적주요문제. 통과재료표면방생미배경적구건개선지가항응성능병가속혈관내피수복시해결상술문제적주요수단. 연구표명,VEGF화SDF-1α균가유효개도혈관내피세포적증식여천이.위비교량류인자재내피화과정중적공능차이,선택재재료표면구건섬련단백/간소공능층,병분별인입VEGF여 SDF-1α량충인자,통과XPS화수접촉각검측연구수식표면적이화성질. 체외동태석방실험결과현시,수식층구유량호적공제인자석방적능력. 체외세포상용성평개결과현시,VEGF화SDF-1α균구유자격내피세포증식화천이적공능,단VEGF표현출경강렬적조절내피세포행위적공능.
Thrombosis and restenosis caused by vascular stent implantation remain major clinical problems.Bio-mimetic microenvironment construction of stent surface to improve the anticoagulant ability and accelerate re-endothelialization has been a main approach to solve above problems.According to recent research, both VEGF and SDF-1αwere found to promote endothelial cells proliferation and migration effectively.To compare the stimulating effect of these two cytokines on endothelialization, VEGF or SDF-1αwas incorporated into the fi-bronectin and heparin modified Ti surface, respectively.XPS and water contact angle assay were used to inves-tigate physical-chemical properties of modified surfaces.The modified surface was found to control VEGF and SDF-1αrelease according to in vitro dynamic release results.In vitro cellular compatibility results indicated that both VEGF and SDF-1αmight contribute to cell proliferation and migration.However, VEGF was found more effective in stimulating cell behavior compared with SDF-1α.