中国药师
中國藥師
중국약사
China Pharmacist
2015年
11期
1987-1988,1989
,共3页
刘彩霞%杨琪%董爱梅%邢玉仁
劉綵霞%楊琪%董愛梅%邢玉仁
류채하%양기%동애매%형옥인
盐酸左氧氟沙星眼用即型凝胶%高效液相色谱法%左氧氟沙星
鹽痠左氧氟沙星眼用即型凝膠%高效液相色譜法%左氧氟沙星
염산좌양불사성안용즉형응효%고효액상색보법%좌양불사성
Levofloxacin hydrochloride ophthalmic in situ gel%HPLC%Levofloxacin
目的：：建立测定盐酸左氧氟沙星眼用即型凝胶中左氧氟沙星含量的HPLC方法。方法：采用Zorbax C18(150 mm ×4.6 mm,5μm)色谱柱,以己烷磺酸钠溶液-甲醇(72∶28)为流动相,流速为1.0 ml·min-1；检测波长为293 nm,柱温为40℃；进样量为10μl。结果：左氧氟沙星在0.0403~0.4030 mg·ml-1范围内线性关系良好(r=1.0000),平均回收率为99.8%(RSD=1.08%,n=9)。结论：该方法准确、简便、快速,适用于测定盐酸左氧氟沙星眼用即型凝胶中左氧氟沙星的含量。
目的：：建立測定鹽痠左氧氟沙星眼用即型凝膠中左氧氟沙星含量的HPLC方法。方法：採用Zorbax C18(150 mm ×4.6 mm,5μm)色譜柱,以己烷磺痠鈉溶液-甲醇(72∶28)為流動相,流速為1.0 ml·min-1；檢測波長為293 nm,柱溫為40℃；進樣量為10μl。結果：左氧氟沙星在0.0403~0.4030 mg·ml-1範圍內線性關繫良好(r=1.0000),平均迴收率為99.8%(RSD=1.08%,n=9)。結論：該方法準確、簡便、快速,適用于測定鹽痠左氧氟沙星眼用即型凝膠中左氧氟沙星的含量。
목적：：건립측정염산좌양불사성안용즉형응효중좌양불사성함량적HPLC방법。방법：채용Zorbax C18(150 mm ×4.6 mm,5μm)색보주,이기완광산납용액-갑순(72∶28)위류동상,류속위1.0 ml·min-1；검측파장위293 nm,주온위40℃；진양량위10μl。결과：좌양불사성재0.0403~0.4030 mg·ml-1범위내선성관계량호(r=1.0000),평균회수솔위99.8%(RSD=1.08%,n=9)。결론：해방법준학、간편、쾌속,괄용우측정염산좌양불사성안용즉형응효중좌양불사성적함량。
Objective:To establish an HPLC method for the determination of levofloxacin in levofloxacin hydrochloride ophthalmic in situ gel. Methods:The chromatographic column was Agilent Zorbax C18(150 mm ×4.6 mm,5μm). The mobile phase consisted of hexane sulfonic acid sodium solution-methanol(72∶28). The flow rate was 1. 0 ml·min-1. The detection wavelength was 293 nm. The chromatographic column temperature was 40℃. The injection volume was 10 μl. Results:The calibration curve was linear within the range of 0. 040 3-0. 403 0 mg·ml-1(r=1. 000 0) for levofloxacin. The average recovery was 99. 8% (RSD=1. 08%,n=9). Conclusion:The method is accurate, simple and rapid, and suitable for the content determination of levofloxacin in levofloxacin hydro-chloride ophthalmic in situ gel.
