中国药师
中國藥師
중국약사
China Pharmacist
2015年
11期
2015-2017
,共3页
间甲酚%含量测定%气相色谱
間甲酚%含量測定%氣相色譜
간갑분%함량측정%기상색보
Metacresol%Content determination%GC
目的：：建立药用辅料间甲酚含量测定的气相色谱分析方法。方法：采用气相色谱法,色谱柱：Agilent DB-225 MS(30 m ×0.25 mm,0.25μm)；升温程序：起始温度为90℃,维持10 min,以每分钟2℃的速率升至120℃,再以每分钟10℃速率升至150℃,维持5 min,进样口温度为200℃,检测器为火焰离子化检测器,温度为250℃。流速为1.8 ml·min-1；分流比为1∶30；载气为氮气。结果：间甲酚在0.6~1.8 mg·ml-1的范围内,其浓度与样品和内标峰面积的比值呈良好线性关系；平均回收率为96.0%,RSD为2.6%(n=9)。三批样品的测量结果分别为99.3%、98.7%和98.4%。结论：所建立的气相色谱法专属性强,准确度高、重复性好,可用于间甲酚的质量控制。
目的：：建立藥用輔料間甲酚含量測定的氣相色譜分析方法。方法：採用氣相色譜法,色譜柱：Agilent DB-225 MS(30 m ×0.25 mm,0.25μm)；升溫程序：起始溫度為90℃,維持10 min,以每分鐘2℃的速率升至120℃,再以每分鐘10℃速率升至150℃,維持5 min,進樣口溫度為200℃,檢測器為火燄離子化檢測器,溫度為250℃。流速為1.8 ml·min-1；分流比為1∶30；載氣為氮氣。結果：間甲酚在0.6~1.8 mg·ml-1的範圍內,其濃度與樣品和內標峰麵積的比值呈良好線性關繫；平均迴收率為96.0%,RSD為2.6%(n=9)。三批樣品的測量結果分彆為99.3%、98.7%和98.4%。結論：所建立的氣相色譜法專屬性彊,準確度高、重複性好,可用于間甲酚的質量控製。
목적：：건립약용보료간갑분함량측정적기상색보분석방법。방법：채용기상색보법,색보주：Agilent DB-225 MS(30 m ×0.25 mm,0.25μm)；승온정서：기시온도위90℃,유지10 min,이매분종2℃적속솔승지120℃,재이매분종10℃속솔승지150℃,유지5 min,진양구온도위200℃,검측기위화염리자화검측기,온도위250℃。류속위1.8 ml·min-1；분류비위1∶30；재기위담기。결과：간갑분재0.6~1.8 mg·ml-1적범위내,기농도여양품화내표봉면적적비치정량호선성관계；평균회수솔위96.0%,RSD위2.6%(n=9)。삼비양품적측량결과분별위99.3%、98.7%화98.4%。결론：소건립적기상색보법전속성강,준학도고、중복성호,가용우간갑분적질량공제。
Objective:To establish a GC method for the determination of metacresol. Methods:The separation was performed on an Agilent DB-225 MS column (30 m × 0. 25 mm,0. 25 μm). The initial column temperature was set at 90℃,maintained 10 min, raised to120℃ with a rate of 2℃·min-1 ,and then raised to 150℃ with a rate of 10℃·min-1 and maintained for 5 min. The inlet temperature was 200℃. The detector was a flame ionization detector (FID) and the temperature was 250℃. The flow rate was 1. 8 ml ·min-1 split ratio was 1∶30 and the carrier gas was nitrogen. Results:The linear range was 0. 6-1. 8 mg·ml-1 . The average recovery was 96. 0% (RSD=2. 6%,n=9). The content of 3 batches of the samples was 99. 3%, 98. 7% and 98. 4%, respectively. Conclu-sion:The established quantitative analysis method is applicable in the quality control of metacresol.
