中国药师
中國藥師
중국약사
China Pharmacist
2015年
11期
1841-1843
,共3页
鲜无蹼壁虎%抗肿瘤活性成分%提取分离
鮮無蹼壁虎%抗腫瘤活性成分%提取分離
선무복벽호%항종류활성성분%제취분리
Fresh Gekko swinhonis%Anti-tumor active ingredient%Extraction and separation
目的：：研究鲜无蹼壁虎抗肿瘤活性成分的提取分离。方法：不同浓度乙醇以不同方法进行提取,提取物经过反复冻融脱蛋白质脱脂肪分离后经高效液相法分离纯化,采用MTT比色法检测其抗肿瘤活性。结果：85%乙醇超声提取工艺Ⅲ组A值为0.548±0.045,抑瘤率最高,与空白对照组比较,差异有统计学意义(P<0.05)。结论：药效学实验结果表明85%乙醇超声提取为最佳提取工艺。
目的：：研究鮮無蹼壁虎抗腫瘤活性成分的提取分離。方法：不同濃度乙醇以不同方法進行提取,提取物經過反複凍融脫蛋白質脫脂肪分離後經高效液相法分離純化,採用MTT比色法檢測其抗腫瘤活性。結果：85%乙醇超聲提取工藝Ⅲ組A值為0.548±0.045,抑瘤率最高,與空白對照組比較,差異有統計學意義(P<0.05)。結論：藥效學實驗結果錶明85%乙醇超聲提取為最佳提取工藝。
목적：：연구선무복벽호항종류활성성분적제취분리。방법：불동농도을순이불동방법진행제취,제취물경과반복동융탈단백질탈지방분리후경고효액상법분리순화,채용MTT비색법검측기항종류활성。결과：85%을순초성제취공예Ⅲ조A치위0.548±0.045,억류솔최고,여공백대조조비교,차이유통계학의의(P<0.05)。결론：약효학실험결과표명85%을순초성제취위최가제취공예。
Objective:To study the extraction and separation of anti-tumor active ingredients from fresh Gekko swinhonis. Meth-ods:The extraction was carried out with ethanol at different concentrations by various methods. The extract was purified by HPLC after deproteinization and degreasing using a repeated freezing and thawing method. MTT was used to detect the antitumor activity of the ex-tract. Results:The A value of extraction process groupⅢwith 85% ethanol ultrasonic extraction was (0. 548 ± 0. 045) with the high-est tumor inhibition rate, and compared with the control group, the difference was significant (P<0. 05). Conclusion:The results of pharmacodynamics show that the optimum extraction technology is ultrasonic extraction with 85% ethanol.
