中国药师
中國藥師
중국약사
China Pharmacist
2015年
11期
1879-1882
,共4页
高效液相色谱法%息喘丸%补骨脂素%佛手柑内酯%去甲蟛蜞菊内酯%蟛蜞菊内酯
高效液相色譜法%息喘汍%補骨脂素%彿手柑內酯%去甲蟛蜞菊內酯%蟛蜞菊內酯
고효액상색보법%식천환%보골지소%불수감내지%거갑팽기국내지%팽기국내지
HPLC%Xichuan pills%Psoralen%Bergapten%Desmethylwedelolactone%Wedelolactone
目的：：建立HPLC法同时测定息喘丸中补骨脂素、佛手柑内酯、去甲蟛蜞菊内酯、蟛蜞菊内酯4个有效成分的含量。方法：采用依利特C18色谱柱(250 mm ×4.6 mm,5μm)；以甲醇-乙腈(2∶1)与0.5%冰醋酸溶液为流动相进行梯度洗脱；流速为1.1 ml·min-1；柱温为25℃；检测波长(0~20 min、在222 nm波长下检测补骨脂素和佛手柑内酯；20~40 min、在351 nm波长下检测去甲蟛蜞菊内酯和蟛蜞菊内酯)。结果：4个有效成分的线性范围分别为：补骨脂素5.380~107.600μg·ml-1( r=0.9995)、佛手柑内酯6.870~137.400μg·ml-1(r=0.9997)、去甲蟛蜞菊内酯4.580~91.600μg·ml-1(r=0.9992)、蟛蜞菊内酯7.300~146.000μg·ml-1(r=0.9999)；平均回收率分别为96.82%、98.81%、99.06%、98.39%,RSD分别为0.77%、1.40%、1.20%、0.80%(n=6)。结论：该方法操作准确稳定、灵敏度高、重复性好,可用于息喘丸中上述4个有效成分的定量测定。
目的：：建立HPLC法同時測定息喘汍中補骨脂素、彿手柑內酯、去甲蟛蜞菊內酯、蟛蜞菊內酯4箇有效成分的含量。方法：採用依利特C18色譜柱(250 mm ×4.6 mm,5μm)；以甲醇-乙腈(2∶1)與0.5%冰醋痠溶液為流動相進行梯度洗脫；流速為1.1 ml·min-1；柱溫為25℃；檢測波長(0~20 min、在222 nm波長下檢測補骨脂素和彿手柑內酯；20~40 min、在351 nm波長下檢測去甲蟛蜞菊內酯和蟛蜞菊內酯)。結果：4箇有效成分的線性範圍分彆為：補骨脂素5.380~107.600μg·ml-1( r=0.9995)、彿手柑內酯6.870~137.400μg·ml-1(r=0.9997)、去甲蟛蜞菊內酯4.580~91.600μg·ml-1(r=0.9992)、蟛蜞菊內酯7.300~146.000μg·ml-1(r=0.9999)；平均迴收率分彆為96.82%、98.81%、99.06%、98.39%,RSD分彆為0.77%、1.40%、1.20%、0.80%(n=6)。結論：該方法操作準確穩定、靈敏度高、重複性好,可用于息喘汍中上述4箇有效成分的定量測定。
목적：：건립HPLC법동시측정식천환중보골지소、불수감내지、거갑팽기국내지、팽기국내지4개유효성분적함량。방법：채용의리특C18색보주(250 mm ×4.6 mm,5μm)；이갑순-을정(2∶1)여0.5%빙작산용액위류동상진행제도세탈；류속위1.1 ml·min-1；주온위25℃；검측파장(0~20 min、재222 nm파장하검측보골지소화불수감내지；20~40 min、재351 nm파장하검측거갑팽기국내지화팽기국내지)。결과：4개유효성분적선성범위분별위：보골지소5.380~107.600μg·ml-1( r=0.9995)、불수감내지6.870~137.400μg·ml-1(r=0.9997)、거갑팽기국내지4.580~91.600μg·ml-1(r=0.9992)、팽기국내지7.300~146.000μg·ml-1(r=0.9999)；평균회수솔분별위96.82%、98.81%、99.06%、98.39%,RSD분별위0.77%、1.40%、1.20%、0.80%(n=6)。결론：해방법조작준학은정、령민도고、중복성호,가용우식천환중상술4개유효성분적정량측정。
Objective:To develop an HPLC method for the simultaneous determination of four active ingredients ( psoralen, ber-gapten, desmethylwedelolactone and wedelolactone) in Xichuan pills. Methods: A Hypersil C18 column was used for the chromato-graphic separation of the four analytes. The mobile phase consisting of methanol-acetonitrile (2∶ 1) and 0. 5% glacial acetic acid solu-tion was used for gradient elution. The flow rate was 1. 1 ml·min-1 . The column temperature was 25℃. Psoralen and bergapten were detected at 222 nm, and desmethylwedelolactone and wedelolactone were detected at 351 nm. Results:There was good linear relation-ship between the concentration of psoralen, bergapten, desmethylwedelolactone and wedelolactone and peak area within the concentra-tion range of 5.380-107.600 μg·ml-1(r =0.999 5),6.870-137.400 μg·ml-1(r =0.999 7),4.580-91.600 μg·ml-1(r =0. 999 2) and 7. 300-146. 000 μg·ml-1(r=0. 999 9),respectively. The average recovery(RSD) was 96. 82%(0. 77%), 98. 81%(1. 40%),99. 06%(1. 20%) and 98. 39% (0. 80%), respectively (n=6). Conclusion:The developed method is accurate, highly sensitive and well reproducible, which can be used for the determination of the four active ingredients in Xichuan pills.
