CAJ | 학술논문

目的：研究 DKK—1（Dickkopf—1）在神经母细胞瘤组织中的表达及临床意义。方法收集本院小儿外科自2010年1月至2015年5月经手术切除的新鲜组织标本，其中神经母细胞瘤（neuro-blastoma，NB）38例，神经节细胞瘤12例。另选取本院病理科保存的正常儿童肾上腺组织11例作为正常对照。用免疫组化法检测 DKK—1在38例神经母细胞瘤，12例神经节细胞瘤及11例正常肾上腺组织中的蛋白表达。结果DKK—1蛋白定位于细胞膜和细胞浆。DKK—1在正常肾上腺组织中呈强阳性表达，在神经节细胞瘤组织中呈弱阳性表达，在神经母细胞瘤组织中呈阴性表达。神经节细胞瘤和神经母细胞瘤中 DKK—1的表达量分别与正常对照组相比，差异均有统计学意义（P ＜0．001）。在3种组织中，不同组间 DKK—1的表达差异有统计学意义（F ＝187．257，P ＜0．001）。结论DKK —1在神经母细胞瘤组织中呈低水平表达，提示其可作为一个特殊的分子标记物，有望应用于神经母细胞瘤的早期诊断及预后监测。
목적：연구 DKK—1（Dickkopf—1）재신경모세포류조직중적표체급림상의의。방법수집본원소인외과자2010년1월지2015년5월경수술절제적신선조직표본，기중신경모세포류（neuro-blastoma，NB）38례，신경절세포류12례。령선취본원병이과보존적정상인동신상선조직11례작위정상대조。용면역조화법검측 DKK—1재38례신경모세포류，12례신경절세포류급11례정상신상선조직중적단백표체。결과DKK—1단백정위우세포막화세포장。DKK—1재정상신상선조직중정강양성표체，재신경절세포류조직중정약양성표체，재신경모세포류조직중정음성표체。신경절세포류화신경모세포류중 DKK—1적표체량분별여정상대조조상비，차이균유통계학의의（P ＜0．001）。재3충조직중，불동조간 DKK—1적표체차이유통계학의의（F ＝187．257，P ＜0．001）。결론DKK —1재신경모세포류조직중정저수평표체，제시기가작위일개특수적분자표기물，유망응용우신경모세포류적조기진단급예후감측。
Objetive To investigate the expression of DKK—1 in pediatric neuroblastoma(NB)tissues. Methods The expressions of DKK—1 in 38 cases of pediatric NB tissues,12 cases of pediatric gangliocytoma tissues and 11 cases of pediatric normal adrenal tissues were detected by immunohistochemical staining. Re-sults DKK—1 protein was localized in the cell membrane and cytoplasm.DKK —1 showed strong positive ex-pression in normal adrenal tissues,weakly positive expression in gangliocytoma tissues and negative expression in NB tissues,the difference was statistically significant(P <0.001 ).The expression differences between three different groups were statistically significant (F =187.257,P <0.001 ).Conclusions The expression of DKK—1 in NB tissues was lower than that of gangliocytoma tissues and normal adrenal tissues,which suggests that DKK—1 may play as a potential molecular marker for early diagnosis and prognosis monitoring in NB.