CAJ | 학술논문

目的：探讨Toll样受体4、7(TLR4、TLR7)蛋白在宫颈鳞癌(CSCC)组织中的表达变化及与患者的临床病理特征的关系。方法选取本院67例确诊CSCC患者的癌组织标本( CSCC组)、30例宫颈上皮瘤变患者的宫颈组织标本( CIN组)及30例正常宫颈组织标本(正常组)，采用免疫组化染色法检测3组标本中的TLR4、TLR7蛋白表达水平差异，并分析CSCC组织标本中TLR4、TLR7蛋白表达与患者的临床病理特征的关系。结果 CSCC组的TLR4、TLR7蛋白表达阳性率分别为80.60%、76.12%，均显著高于CIN组的46.67%、40.00%和正常组的13.33%、10.00%，且差异均具有统计学意义(P<0.05)；CIN组的TLR4、TLR7蛋白阳性表达率显著高于正常组(P<0.05)。 TLR4蛋白阳性表达与CSCC 患者的FIGO分期、病理分级、浸润深度有显著的关系(P<0.05)；TLR7蛋白阳性表达与CSCC 患者的病理分级、浸润深度有显著的关系(P<0.05)。结论 TLR4、TLR7蛋白在CSCC患者宫颈组织中表达阳性率显著升高，并且与患者的临床分期、病理分级等因素有一定的关系。
목적：탐토Toll양수체4、7(TLR4、TLR7)단백재궁경린암(CSCC)조직중적표체변화급여환자적림상병리특정적관계。방법선취본원67례학진CSCC환자적암조직표본( CSCC조)、30례궁경상피류변환자적궁경조직표본( CIN조)급30례정상궁경조직표본(정상조)，채용면역조화염색법검측3조표본중적TLR4、TLR7단백표체수평차이，병분석CSCC조직표본중TLR4、TLR7단백표체여환자적림상병리특정적관계。결과 CSCC조적TLR4、TLR7단백표체양성솔분별위80.60%、76.12%，균현저고우CIN조적46.67%、40.00%화정상조적13.33%、10.00%，차차이균구유통계학의의(P<0.05)；CIN조적TLR4、TLR7단백양성표체솔현저고우정상조(P<0.05)。 TLR4단백양성표체여CSCC 환자적FIGO분기、병리분급、침윤심도유현저적관계(P<0.05)；TLR7단백양성표체여CSCC 환자적병리분급、침윤심도유현저적관계(P<0.05)。결론 TLR4、TLR7단백재CSCC환자궁경조직중표체양성솔현저승고，병차여환자적림상분기、병리분급등인소유일정적관계。
Objective To study the changes of Toll like receptor 4,7 (TLR4,TLR7) protein in cervical squamous cell carcinoma ( CSCC) tissues and the relationship between expression and clinical pathological characteristics of the patients. Methods A total of 67 pa-tients with CSCC carcinoma( CSCC group) ,30 patients with cervical intraepithelial neoplasia( CIN group) and 30 patients with normal cervi-cal tissues(normal group) were detected by immunohistochemical method. The expression of TLR4,TLR7 protein and its clinical pathological characteristics of patients with CSCC in the samples were analyzed. Results TLR4,TLR7 protein positive rate of CSCC group were respec-tively 80. 60% and 76. 12%,which were significantly higher than those of CIN group (46. 67%,40. 00%) and the normal group(13. 33%, 10. 00%),the difference was statistically significant (P<0. 05). TLR4,TLR7 protein positive expression rate in CIN group was significantly higher than that in the normal group (P<0. 05). The positive expression of TLR4 protein and CSCC in patients with FIGO stage,pathological grade,depth of invasion had a significant relationship(P<0. 05),pathological grade,the positive expression of TLR7 protein and CSCC in pa-tients with invasive depth had a significant relationship(P<0. 05). Conclusion There is increased expression of TLR4,TLR7 protein in cervical tissues of CSCC patients,and a certain relationship between the factors and clinical staging,pathological grading.