CAJ | 학술논문

目的：分别检测重度子痫前期和正常妊娠妇女胎盘组织中微小 RNA（miRNA）的表达情况，并初步探讨miRNA 的差异表达在重度子痫前期发生发展过程中的意义。方法在我院住院剖宫产分娩的产妇中，选取5例重度子痫前期患者（实验组），5例正常妊娠孕妇（对照组），通过 miRNA 芯片技术检测实验组与对照组胎盘组织中 miRNA的表达情况。结果本实验共检测了391个 miRNA 的表达谱，实验组与对照组组间比较，差异表达的 miRNA 共有81个，其中表达上调的有80个，表达下调的有1个，主要涉及细胞分化、血管重塑、内分泌调节等多方面的功能。结论重度子痫前期患者胎盘组织中 miRNA 的表达失调可能参与了重度子痫前期的发病及病理生理过程。
목적：분별검측중도자간전기화정상임신부녀태반조직중미소 RNA（miRNA）적표체정황，병초보탐토miRNA 적차이표체재중도자간전기발생발전과정중적의의。방법재아원주원부궁산분면적산부중，선취5례중도자간전기환자（실험조），5례정상임신잉부（대조조），통과 miRNA 심편기술검측실험조여대조조태반조직중 miRNA적표체정황。결과본실험공검측료391개 miRNA 적표체보，실험조여대조조조간비교，차이표체적 miRNA 공유81개，기중표체상조적유80개，표체하조적유1개，주요섭급세포분화、혈관중소、내분비조절등다방면적공능。결론중도자간전기환자태반조직중 miRNA 적표체실조가능삼여료중도자간전기적발병급병리생리과정。
Objective To detect differential expression of small RNA (miRNA)in placental tissue from severe pre-eclampsia and normal maternal,and to explore the pathogenesis of severe preeclampsia.Methods We chose 5 cases of severe preeclampsia patients (experimental group)and 5 cases of normal pregnancy pregnant women (control group)a-mong those delivery in hospital.And the miRNA expression profile was studied using miRNA microarray analysis.Re-sults Eighty-one miRNAs were found to be dysregulated in placentas of sPE patients among the 391 identified genes. Eighty miRNAs were overexpressed and only one miRNA was underexpressed.These differential expression of miRNA may be related to cell differentiation,vascular remodeling,endocrine regulation,and so on.Conclusion The dysregula-tion of miRNA in severe preeclamptic placental tissue is closely associated with the the pathophysiological process of sPE.