中国药物应用与监测
中國藥物應用與鑑測
중국약물응용여감측
Chinese Journal of Drug Application and Monitoring
2015年
5期
271-274
,共4页
马萍%吴诚%王宇%王景成%曹淼%孙平%牛华英
馬萍%吳誠%王宇%王景成%曹淼%孫平%牛華英
마평%오성%왕우%왕경성%조묘%손평%우화영
氯柳酊%氯霉素%水杨酸%高效液相色谱法%含量测定
氯柳酊%氯黴素%水楊痠%高效液相色譜法%含量測定
록류정%록매소%수양산%고효액상색보법%함량측정
Tincture%Chloramphenicol%Salicylic acid%HPLC%Content determination
目的:建立HPLC法同时测定氯柳酊中氯霉素和水杨酸的含量.方法:色谱柱为C18柱(4.6 mm×250 mm,5μm);流动相为甲醇-水-冰醋酸(50:50:1);流速为1.0 mL·min-1;柱温为30℃;检测波长为295 nm;进样量为10 μL.结果:氯霉素和水杨酸分离良好.氯霉素在60.77 ~ 141.80 μg·mL-1范围内线性关系良好(r = 0.999 8),水杨酸在123.50 ~ 288.10 μg·mL-1范围内线性关系良好(r = 0.999 7),氯霉素和水杨酸的平均回收率分别为99.76%、99.87%,RSD分别为1.08%(n = 9)、1.19%(n = 9).结论:本方法简便准确,快速可靠,可用于氯柳酊的含量测定和质量控制.
目的:建立HPLC法同時測定氯柳酊中氯黴素和水楊痠的含量.方法:色譜柱為C18柱(4.6 mm×250 mm,5μm);流動相為甲醇-水-冰醋痠(50:50:1);流速為1.0 mL·min-1;柱溫為30℃;檢測波長為295 nm;進樣量為10 μL.結果:氯黴素和水楊痠分離良好.氯黴素在60.77 ~ 141.80 μg·mL-1範圍內線性關繫良好(r = 0.999 8),水楊痠在123.50 ~ 288.10 μg·mL-1範圍內線性關繫良好(r = 0.999 7),氯黴素和水楊痠的平均迴收率分彆為99.76%、99.87%,RSD分彆為1.08%(n = 9)、1.19%(n = 9).結論:本方法簡便準確,快速可靠,可用于氯柳酊的含量測定和質量控製.
목적:건립HPLC법동시측정록류정중록매소화수양산적함량.방법:색보주위C18주(4.6 mm×250 mm,5μm);류동상위갑순-수-빙작산(50:50:1);류속위1.0 mL·min-1;주온위30℃;검측파장위295 nm;진양량위10 μL.결과:록매소화수양산분리량호.록매소재60.77 ~ 141.80 μg·mL-1범위내선성관계량호(r = 0.999 8),수양산재123.50 ~ 288.10 μg·mL-1범위내선성관계량호(r = 0.999 7),록매소화수양산적평균회수솔분별위99.76%、99.87%,RSD분별위1.08%(n = 9)、1.19%(n = 9).결론:본방법간편준학,쾌속가고,가용우록류정적함량측정화질량공제.
Objective:To establish the HPLC method for simultaneous determination of chloramphenicol and salicylic acid in tincture.Methods: HPLC was used in the quantitative analysis with C18 chromatographic column (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of methanol-water-glacial acetic acid (50 : 50 : 1) with the flow rate of 1.0 mL·min-1. The column temperature was 30℃. The detection wavelength was 295 nm and the injection volume was 10 μL.Results:The calibration curves of chloramphenicol and salicylic acid had a good linearity in the range of 60.77– 141.80 μg·mL-1 (r = 0.999 8)and 123.50– 288.10 μg·mL-1 (r = 0.999 7)respectively. The average recovery of chloramphenicol was 99.76% with RSD of 1.08% (n = 9). The average recovery of salicylic acid was 99.87% with RSD of 1.19% (n = 9).Conclusion:The method is simple, accurate and reliable, which can be used as the quality control method of this preparation.
