CAJ | 학술논문

目的:建立高效液相色谱法测定血塞通软胶囊中三七皂苷R1、人参皂苷Rg1、Re、Rb1含量的方法,为制定该制剂质量标准提供参考.方法:采用HPLC法,选用ZORBAX SB-Aq色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-水梯度洗脱;流速:1.0 mL·min-1;检测波长:203 nm;柱温:25℃.结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1分别在0.32 ~ 3.20μg、0.72 ~ 7.20μg、0.21 ~ 2.10μg、0.92 ~ 9.20μg范围内呈良好线性关系;相关系数分别为1.000 0、1.000 0、0.999 9、1.000 0;平均加样回收率(n = 9)分别为100.22%、100.62%、100.02%、100.33%,且RSD分别为0.99%、1.18%、2.02%、0.97%.结论:该方法简便可行、准确、可靠,重现性好,结果稳定,可为血塞通软胶囊的质量控制方法提供参考.
목적:건립고효액상색보법측정혈새통연효낭중삼칠조감R1、인삼조감Rg1、Re、Rb1함량적방법,위제정해제제질량표준제공삼고.방법:채용HPLC법,선용ZORBAX SB-Aq색보주(250 mm×4.6 mm,5μm);류동상위을정-수제도세탈;류속:1.0 mL·min-1;검측파장:203 nm;주온:25℃.결과:삼칠조감R1、인삼조감Rg1、인삼조감Re화인삼조감Rb1분별재0.32 ~ 3.20μg、0.72 ~ 7.20μg、0.21 ~ 2.10μg、0.92 ~ 9.20μg범위내정량호선성관계;상관계수분별위1.000 0、1.000 0、0.999 9、1.000 0;평균가양회수솔(n = 9)분별위100.22%、100.62%、100.02%、100.33%,차RSD분별위0.99%、1.18%、2.02%、0.97%.결론:해방법간편가행、준학、가고,중현성호,결과은정,가위혈새통연효낭적질량공제방법제공삼고.
Objective:To establish the method for determining the notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Xuesaitong soft capsules by HPLC.Methods: The separation was performed on ZORBAX SB-Aq column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water with linear gradient elution. The flow rate was 1.0 mL·min-1. The detection wavelength was 203 nm and the column temperature was 25℃.Results: The calibration curves showed good linearity in the range of 0.32–3.20 μg (notoginsenoside R1,r = 1.000 0), 0.72– 7.20 μg (ginsenoside Rg1,r = 1.000 0), 0.21– 2.10 μg (ginsenoside Re,r = 0.999 9), 0.92– 9.20 μg (ginsenoside Rb1,r = 1.000 0). The average recoveries (n = 9) were 100.22%, 100.62%, 100.02%, 100.33% respectively, and RSD were 0.99%, 1.18%, 2.02%, 0.97% respectively.Conclusion: The method is convenient, rapid, economical and accurate, which is suitable for quality control of Xuesaitong soft capsules.