CAJ | 학술논문

用金属注射成形(MIM)技术制备孔隙度为60%的多孔钛,用改良冷凝聚合交联法制备明胶缓释微球并涂覆于多孔钛表面,体外细胞评价胰岛素生长因子-1(IGF-1)、转化生长因子- β1(TGF- β1)明胶缓释微球涂层多孔钛对MG63细胞功能的影响.结果表明:明胶缓释微球涂层多孔钛无细胞毒性;当IGF-1、TGF- β1明胶缓释微球的载药浓度分别在0.1~10 ng/mg和0.25~2.5 ng/mg范围内时,与MG63细胞共培养,IGF-1和TGF- β1的载药浓度与细胞的增殖和分化呈正相关;当微球的载药浓度IGF-1为10 ng/mg,TGF- β1为2.5 ng/mg时,MG63细胞具有最优的增殖和分化;IGF-1和TGF- β1联合应用对MG63细胞的黏附、增殖与分化作用明显优于单一应用.
용금속주사성형(MIM)기술제비공극도위60%적다공태,용개량냉응취합교련법제비명효완석미구병도복우다공태표면,체외세포평개이도소생장인자-1(IGF-1)、전화생장인자- β1(TGF- β1)명효완석미구도층다공태대MG63세포공능적영향.결과표명:명효완석미구도층다공태무세포독성;당IGF-1、TGF- β1명효완석미구적재약농도분별재0.1~10 ng/mg화0.25~2.5 ng/mg범위내시,여MG63세포공배양,IGF-1화TGF- β1적재약농도여세포적증식화분화정정상관;당미구적재약농도IGF-1위10 ng/mg,TGF- β1위2.5 ng/mg시,MG63세포구유최우적증식화분화;IGF-1화TGF- β1연합응용대MG63세포적점부、증식여분화작용명현우우단일응용.
Porous titanium with porosity of 60% was prepared by metal injection molding (MIM), and coated with gelatin sustained-release microspheres which were made by improved emulsified cold condensation method. The effects of porous titanium coated with insulin-like growth factor-1 (IGF-1) and transforming growth factor- β1 (TGF- β1) gelatin microspheres on the function of MG63 cells were evaluated in vitro. The results show that porous titanium coated with gelatin sustained-release microspheres has no cytotoxicity. The IGF-1 and TGF- β1 loading concentrations are positively correlative with the proliferation and differentiation of MG63 after co-culturing with the concentrations of IGF-1 and TGF- β1 gelatin microspheres in the range of 0.1?10 ng/mg and 0.25?2.5 ng/mg, respectively. The MG63 cells exhibit the best proliferation and differentiation with the IGF-1 and TGF- β1 loading concentrations of 10 ng/mg and 2.5 ng/mg, respectively. The joint application of IGF-1 and TGF- β1 group, which promote adhesion, proliferation and differentiation of MG63 cells, is superior to a single application group.