CAJ | 학술논문

研究典型中温嗜酸菌株Acidithiobacillus ferrooxidans ATCC 23270对两种不同形态单质硫( α-S8和μ-S)的利用和硫形态转化.A. ferrooxidans分别在 α-S8或者μ-S中培养以适应能源底物.A. ferrooxidans在这两种单质硫中的生长和硫氧化行为的结果表明,A. ferrooxidans在 α-S8中生长时比在μ-S中生长晚1 d进入对数期,但是A. ferrooxidans在 α-S8中生长时在对数期生长较快且培养液具有较高的细胞浓度和较低的pH值.经过A. ferrooxidans的培养,这两种单质硫都被明显腐蚀和细胞修饰.此外,经过A. ferrooxidans的生长,无定型态的μ-S表面组分变为63.1%μ-S和36.9% α-S8,正交晶系的 α-S8表面组分变为68.3% α-S8和31.7%μ-S;而无菌对照组μ-S和 α-S8表面组分都没有变化,表明这两种不同形态的单质硫在A. ferrooxidans的作用下发生了相互转化.
연구전형중온기산균주Acidithiobacillus ferrooxidans ATCC 23270대량충불동형태단질류( α-S8화μ-S)적이용화류형태전화.A. ferrooxidans분별재 α-S8혹자μ-S중배양이괄응능원저물.A. ferrooxidans재저량충단질류중적생장화류양화행위적결과표명,A. ferrooxidans재 α-S8중생장시비재μ-S중생장만1 d진입대수기,단시A. ferrooxidans재 α-S8중생장시재대수기생장교쾌차배양액구유교고적세포농도화교저적pH치.경과A. ferrooxidans적배양,저량충단질류도피명현부식화세포수식.차외,경과A. ferrooxidans적생장,무정형태적μ-S표면조분변위63.1%μ-S화36.9% α-S8,정교정계적 α-S8표면조분변위68.3% α-S8화31.7%μ-S;이무균대조조μ-S화 α-S8표면조분도몰유변화,표명저량충불동형태적단질류재A. ferrooxidans적작용하발생료상호전화.
The utilization and speciation transformation of α-S8andμ-S by the typical mesophilic acidophilic strainAcidithiobacillus ferrooxidans ATCC 23270 were investigated.A. ferrooxidans cells first acclimated to the energy source α-S8 orμ-S, respectively. The results of cell growth and sulfur oxidation behavior showed that the strain grown on α-S8 entered slowly (about 1 d later) into the exponential phase, while grew faster in the exponential phase and attained higher maximal cell density and lower pH value than that onμ-S. After bio-corrosion, both of the two sulfur samples were evidently eroded and modified byA. ferrooxidans cells. After growth ofA. ferrooxidans, the surface composition of amorphousμ-S became 63.1%μ-S and 36.9% α-S8, and that of orthorhombic α-S8became 68.3% α-S8 and 31.7%μ-S, while the surface compositions of α-S8 andμ-S in sterile experiment were not changed, indicating that these two elemental sulfur species can be interconverted byA. ferrooxidans.