CAJ | 학술논문

目的：探讨 DC（dendritic cells）、CIK（cytokine-induced killer cells）联合培养对肺腺癌细胞 A549的杀伤活性的影响，并与单独 CIK 对 A549杀伤活性进行比较，为肿瘤生物治疗提供有效方法。方法分别培养 DC、CIK 细胞，经鉴定后进行联合培养，联合培养3天后，对 A549细胞进行杀伤活性实验，检测 DC 联合 CIK 及单独 CIK 对A549的杀伤活性，同时用流式细胞检测 DC、CIK 联合培养细胞及单独 CIK 细胞中淋巴细胞亚群的表达。结果DC联合 CIK 对 A549的杀伤活性为88．38％，CIK 对 A549的杀伤活性为66．24％，两者对 A549的杀伤活性比较有显著差异（P ＜0．01）；DC、CIK 联合培养的淋巴细胞亚群中的 CD3＋、CD4＋、NK 、NKT 、CD3＋ HLA-DR＋、CD8＋ CD28＋的数量均高于 CIK 细胞。结论DC 、CIK 联合培养对肺癌细胞 A549的杀伤活性明显高于单独 CIK 对 A549的杀伤活性（P ＜0．01）；抗肿瘤淋巴细胞（CD3＋、CD4＋、NK、NKT 、CD3＋ HLA-DR＋、CD8＋ CD28＋）数量也明显高于 CIK 细胞。
목적：탐토 DC（dendritic cells）、CIK（cytokine-induced killer cells）연합배양대폐선암세포 A549적살상활성적영향，병여단독 CIK 대 A549살상활성진행비교，위종류생물치료제공유효방법。방법분별배양 DC、CIK 세포，경감정후진행연합배양，연합배양3천후，대 A549세포진행살상활성실험，검측 DC 연합 CIK 급단독 CIK 대A549적살상활성，동시용류식세포검측 DC、CIK 연합배양세포급단독 CIK 세포중림파세포아군적표체。결과DC연합 CIK 대 A549적살상활성위88．38％，CIK 대 A549적살상활성위66．24％，량자대 A549적살상활성비교유현저차이（P ＜0．01）；DC、CIK 연합배양적림파세포아군중적 CD3＋、CD4＋、NK 、NKT 、CD3＋ HLA-DR＋、CD8＋ CD28＋적수량균고우 CIK 세포。결론DC 、CIK 연합배양대폐암세포 A549적살상활성명현고우단독 CIK 대 A549적살상활성（P ＜0．01）；항종류림파세포（CD3＋、CD4＋、NK、NKT 、CD3＋ HLA-DR＋、CD8＋ CD28＋）수량야명현고우 CIK 세포。
Objective To discussion of cytotoxicity effects of DC (dendritic cells)and CIK (cytokine-induced cells) co-cultured on lung adenocarcinoma cell line A549,and Compare with CIK alone on A549 killing activity,Provide an ef-fective method for tumor biotherapy.Methods respectively cultured DC,CIK cell,and co-cultured after the identifica-tion,after 3 days,to killing activity experiments on A549 cell,detect cytotoxicity of DC joint CIK and separate CIK a-gainst A549,While using flow cytometry detect lymphocyte subsets expression of DC joint CIK and CIK alone.Results The cytotoxicity of DC joint CIK on A549 was 88.38%,CIK was 66.24%,there were significant differences of cyto-toxic activity on A549(P <0.01)between,the number of lymphocyte subsets (CD3 + ,CD4 + ,NK,NKT,CD3 + HLA-DR+ ,CD8+ CD28+ cell)of DC joint CIK was significantly higher than CIK.Conclusion The killing activity of DC and CIK co-cultured on A549 lung cancer has significantly higher than that of single CIK cytotoxicity on A549(P <0.01).