中国实验诊断学
中國實驗診斷學
중국실험진단학
Chinese Journal of Laboratory Diagnosis
2015年
10期
1651-1654
,共4页
高尔基体蛋白 73%甲胎蛋白异质体 3%磷脂酰肌醇蛋白聚糖-3%原发性肝癌%病毒性肝炎%肝硬化
高爾基體蛋白 73%甲胎蛋白異質體 3%燐脂酰肌醇蛋白聚糖-3%原髮性肝癌%病毒性肝炎%肝硬化
고이기체단백 73%갑태단백이질체 3%린지선기순단백취당-3%원발성간암%병독성간염%간경화
Golgi protein 73%Alpha fetoprotein heterogeneity 3%Glypican-3%Primary hepatic carcinoma%Hepatitis B virus%Liver cirrhosis
目的：探讨高尔基体蛋白73（GP73）、甲胎蛋白异质体3（AFP-L3）和磷脂酰肌醇蛋白聚糖-3（GPC-3）联合检测在原发性肝癌（PHC）诊断中的临床价值。方法采用免疫组织化学法和酶联免疫法检测原发性肝癌（PHC 组，n＝34例）、肝硬化（LC 组，n＝25例）和病毒性肝炎（VH 组，n＝27例）患者肝组织和血清 GP73、AFP-L3和 GPC-3的表达水平，同期选择30例门诊正常体检者作为对照组。观察各组肝组织和血清 GP73、AFP-L3和 GPC-3的表达水平，并比较 GP73、AFP-L3和 GPC-3单一和联合检测 PHC 的阳性率。结果PHC 组肝组织及血清 AFP-L3、GP73和GPC-3表达水平均明显高于其他各组（P ＜0．05），3项标志物（AFP-L3、GP73和 GPC-3）单项检测 PHC 的阳性率分别为70．6％、76．5％、79．4％，两两指标联合检测 PHC 的阳性率可至82．3％、85．3％和88．2％，3项指标联合检测 PHC的阳性率可高达91．2％，明显高于单项检测率（P ＜0．05）。结论AFP-L3、GP73和 GPC-3联合检测可提高 PHC 阳性率，对早期 PHC 诊断具有重要的临床意义。
目的：探討高爾基體蛋白73（GP73）、甲胎蛋白異質體3（AFP-L3）和燐脂酰肌醇蛋白聚糖-3（GPC-3）聯閤檢測在原髮性肝癌（PHC）診斷中的臨床價值。方法採用免疫組織化學法和酶聯免疫法檢測原髮性肝癌（PHC 組，n＝34例）、肝硬化（LC 組，n＝25例）和病毒性肝炎（VH 組，n＝27例）患者肝組織和血清 GP73、AFP-L3和 GPC-3的錶達水平，同期選擇30例門診正常體檢者作為對照組。觀察各組肝組織和血清 GP73、AFP-L3和 GPC-3的錶達水平，併比較 GP73、AFP-L3和 GPC-3單一和聯閤檢測 PHC 的暘性率。結果PHC 組肝組織及血清 AFP-L3、GP73和GPC-3錶達水平均明顯高于其他各組（P ＜0．05），3項標誌物（AFP-L3、GP73和 GPC-3）單項檢測 PHC 的暘性率分彆為70．6％、76．5％、79．4％，兩兩指標聯閤檢測 PHC 的暘性率可至82．3％、85．3％和88．2％，3項指標聯閤檢測 PHC的暘性率可高達91．2％，明顯高于單項檢測率（P ＜0．05）。結論AFP-L3、GP73和 GPC-3聯閤檢測可提高 PHC 暘性率，對早期 PHC 診斷具有重要的臨床意義。
목적：탐토고이기체단백73（GP73）、갑태단백이질체3（AFP-L3）화린지선기순단백취당-3（GPC-3）연합검측재원발성간암（PHC）진단중적림상개치。방법채용면역조직화학법화매련면역법검측원발성간암（PHC 조，n＝34례）、간경화（LC 조，n＝25례）화병독성간염（VH 조，n＝27례）환자간조직화혈청 GP73、AFP-L3화 GPC-3적표체수평，동기선택30례문진정상체검자작위대조조。관찰각조간조직화혈청 GP73、AFP-L3화 GPC-3적표체수평，병비교 GP73、AFP-L3화 GPC-3단일화연합검측 PHC 적양성솔。결과PHC 조간조직급혈청 AFP-L3、GP73화GPC-3표체수평균명현고우기타각조（P ＜0．05），3항표지물（AFP-L3、GP73화 GPC-3）단항검측 PHC 적양성솔분별위70．6％、76．5％、79．4％，량량지표연합검측 PHC 적양성솔가지82．3％、85．3％화88．2％，3항지표연합검측 PHC적양성솔가고체91．2％，명현고우단항검측솔（P ＜0．05）。결론AFP-L3、GP73화 GPC-3연합검측가제고 PHC 양성솔，대조기 PHC 진단구유중요적림상의의。
Objective To investigate the clinical value of Golgi protein 73 (GP73),alpha fetoprotein heterogeneity 3 (AFP-L3)combined Glypican-3(GPC-3)detection with in the diagnosis of primary hepatic carcinoma.Methods The expression levels of GP73,AFP-L3 and GPC-3 in liver tissue and serum of primary hepatic carcinoma (group PHC,n=34),liver cirrhosis (group LC,n=25)and viral hepatitis (group VH,n=27)patients were detected by immunohisto-chemical and enzyme immunoassay.At the same period,30 cases of normal examination were selected as control group. The expression levels of GP73,AFP-L3 and GPC-3 in liver tissue and serum of all groups were observed,and compared the positive rate of GP73,AFP-L3,GPC-3 single detection and combined detection to primary hepatic carcinoma.Results The expression levels of GP73,AFP-L3 and GPC-3 in the liver tissue and serum of PHC group were significantly higher than that of other groups (P <0.05).The single test positive rate of AFP-L3,GP73 and GPC-3 were 70.6%, 76.5%,79.4% to PHC,two indicators combined detection of PHC were 82.3%,85.3% and 88.2%,3 indicators com-bined detection of PHC was 91.2%.The rate of 3 indicators combined detection was higher than that of single indicator (P <0.05).Conclusion The combined detection of AFP-L3,GP73 and GPC-3 could improve the positive rate of PHC. It had an important clinical significance for early diagnosis of PHC.
