CAJ | 학술논문

目的：探讨凝血酶预处理(thrombin preconditioning,TPC)对大鼠脑出血后内源性的神经细胞再生的影响。方法SD 大鼠随机分为对照组、脑出血(Intracerebral hemorrhage,ICH)组和 TPC 组,每组分为3、7、14、21、28 d 亚组。应用胶原酶脑内立体定向注射制作脑出血模型。TPC 组首先将1 U 凝血酶立体定向注入右侧纹状体,1 d 后再制作脑出血模型。所有大鼠应用 BrdU 腹腔注射在体标记再生的脑室下带(sub-ventricular zone,SVZ)细胞,应用免疫组织化学技术检测 BrdU 阳性细胞。结果脑出血后3 d 同侧 SVZ 和基底节 BrdU 阳性细胞开始增加,但与对照组比较差异没有显著性(P >0.05),7 d 时 BrdU 阳性细胞数增加明显,与对照组比较有明显差异(P <0.05),14 d 达到高峰,然后逐渐下降,28 d 时 BrdU 阳性细胞虽然仍有增加,但与对照组比较差异没有显著性(P >0.05)。TPC 组3 d 时 BrdU 阳性细胞数目开始增加,与脑出血组和对照组比较,差异均具有显著性(P <0.01),14 d 达高峰,一直持续至21 d,28 d 时 BrdU 阳性细胞虽然略有下降,但与对照组和脑出血组比较,仍有统计学差异(P <0.05)。结论凝血酶预处理能够增强脑出血后内源性神经再生,可能为脑出血后内源性神经再生的研究提供新的思路。
목적：탐토응혈매예처리(thrombin preconditioning,TPC)대대서뇌출혈후내원성적신경세포재생적영향。방법SD 대서수궤분위대조조、뇌출혈(Intracerebral hemorrhage,ICH)조화 TPC 조,매조분위3、7、14、21、28 d 아조。응용효원매뇌내입체정향주사제작뇌출혈모형。TPC 조수선장1 U 응혈매입체정향주입우측문상체,1 d 후재제작뇌출혈모형。소유대서응용 BrdU 복강주사재체표기재생적뇌실하대(sub-ventricular zone,SVZ)세포,응용면역조직화학기술검측 BrdU 양성세포。결과뇌출혈후3 d 동측 SVZ 화기저절 BrdU 양성세포개시증가,단여대조조비교차이몰유현저성(P >0.05),7 d 시 BrdU 양성세포수증가명현,여대조조비교유명현차이(P <0.05),14 d 체도고봉,연후축점하강,28 d 시 BrdU 양성세포수연잉유증가,단여대조조비교차이몰유현저성(P >0.05)。TPC 조3 d 시 BrdU 양성세포수목개시증가,여뇌출혈조화대조조비교,차이균구유현저성(P <0.01),14 d 체고봉,일직지속지21 d,28 d 시 BrdU 양성세포수연략유하강,단여대조조화뇌출혈조비교,잉유통계학차이(P <0.05)。결론응혈매예처리능구증강뇌출혈후내원성신경재생,가능위뇌출혈후내원성신경재생적연구제공신적사로。
Objective To identify the effect of thrombin preconditioning (TPC)on endogenous neuro-genesis after intracerebral hemorrhage (ICH)in rats.Methods Sprague-Dawley rats were randomly divided into control,ICH and TPC groups.Each group was divided into 3d,7d,14d,21 d,and 28d subgroups respec-tively.ICH model was induced by collagenase stereotaxical injection.In TPC group,1 U thrombin was ster-eotaxically injected into basal ganglia area 1 day before ICH model was conducted.SVZ cells were marked by BrdU intraperitoneal injection.BrdU positive cells were detected by immunohistochemical methods.Results BrdU positive cells increased at 3 d in the ipsilateral SVZ and basal ganglia area after ICH,and there was no significant difference compared with control group (P >0.05).BrdU positive cells were remarkably increased at 7 d with significant difference compared to control group (P >0.05),peaked at 14d and then declined.Br-dU positive cells were still increased at 28 d after ICH,and there was no significant difference compared with control group (P >0.05).BrdU positive cells were increased at 3 d in the ipsilateral SVZ and basal ganglia ar-ea in TPC group with significant difference compared with control and ICH groups (P <0.01),peaked at 7 d and continued to the 21 d.BrdU positive cells were slightly decreased at 28 d in the ipsilateral SVZ and basal ganglia area in TPC group,but there was still significant difference compared with control and ICH groups (P<0.01 ).Conclusions Thrombin preconditioning can enhance endogenous neurogenesis after intracerebral hemorrhage,which may provide new ideas for the study of endogenous neurogenesis after intracerebral hemor-rhage.