CAJ | 학술논문

目的:通过蛋白质组学方法,以人乳腺癌MDA-MB-435细胞为对象,筛查红景天苷干预所致的差异表达蛋白质,为后续研究红景天苷治疗乳腺癌的分子机制提供初步依据. 方法:用双向电泳、软件分析和质谱分析等常用蛋白质组学技术检测4 mg/mL的红景天苷对MDA-MB-435细胞蛋白质表达谱的影响. 结果:获得了红景天苷作用MDA-MB-435细胞的双向电泳图谱,初步鉴定了6个与红景天苷作用相关的差异表达蛋白质,包括细胞质肌动蛋白1、膜联蛋白A5、原肌球蛋白3亚型CRA c、原肌球蛋白α-4链亚型2、原肌球蛋白3亚型CRA e、酪氨酸3-单加氧酶/色氨酸.结论:成功建立了红景天苷作用于MDA-MB-435细胞的蛋白质组双向电泳分离体系,筛选出6个与红景天苷作用相关的差异表达蛋白质.
목적:통과단백질조학방법,이인유선암MDA-MB-435세포위대상,사사홍경천감간예소치적차이표체단백질,위후속연구홍경천감치료유선암적분자궤제제공초보의거. 방법:용쌍향전영、연건분석화질보분석등상용단백질조학기술검측4 mg/mL적홍경천감대MDA-MB-435세포단백질표체보적영향. 결과:획득료홍경천감작용MDA-MB-435세포적쌍향전영도보,초보감정료6개여홍경천감작용상관적차이표체단백질,포괄세포질기동단백1、막련단백A5、원기구단백3아형CRA c、원기구단백α-4련아형2、원기구단백3아형CRA e、락안산3-단가양매/색안산.결론:성공건립료홍경천감작용우MDA-MB-435세포적단백질조쌍향전영분리체계,사선출6개여홍경천감작용상관적차이표체단백질.
To investigate the differentially expressed proteins after treatment of human breast cancer MDA-MB-435 cells with Salidroside, which may help us to elucidate the molecular mechanism of Salidroside in the treatment of human breast cancer. Methods: The proteomics technologies such as 2-DE,software analysis, and MS were used to investigate 2-DE profiling on MDA-MB-435 cells treated with 4 mg/mL Salidroside. Results: 2-DE profilings in MDA-MB-435 cells treated with Salidroside were established, and six differentially expressed proteins were identified as actin, cytoplasmic 1,annexin A5,tropomyosin 3 isoform CRA c,tropomyosinalpha-4chainisoform2,tropomyosin3isoformCRA e,andtyrosine3-monooxygenase/tryptophan. Conclusion: 2-DE separation system for proteomes from MDA-MB-435 cells treated with Salidroside has been successfully established,and six differentially expressed proteins were identified.