泸州医学院学报
瀘州醫學院學報
로주의학원학보
Journal of Luzhou Medical College
2015年
5期
437-440
,共4页
黄娟%刘浩%曾凡才%陈明%刘萍%莫冬阳%李鑫
黃娟%劉浩%曾凡纔%陳明%劉萍%莫鼕暘%李鑫
황연%류호%증범재%진명%류평%막동양%리흠
BIOMED-2系统%非霍奇金淋巴瘤%B细胞%抗原受体%基因重排
BIOMED-2繫統%非霍奇金淋巴瘤%B細胞%抗原受體%基因重排
BIOMED-2계통%비곽기금림파류%B세포%항원수체%기인중배
BIOMED-2 system%Non-Hodgkin lymphoma%B-cell%antigen receptor%Gene rearrangement
目的:探讨BIOMED-2系统在检测B细胞非霍奇金淋巴瘤中的应用价值.方法:选取石蜡包埋组织B细胞非霍奇金淋巴瘤40例、其他淋巴结增生性病变10例,提取组织DNA,应用BIOMED-2系统中的61条引物分成9个组别进行PCR扩增,检测DNA质量及抗原受体基因重排克隆性.结果:94%(47/50)样本DNA长度在300 bp以上,BIOMED-2系统检测的敏感性和特异性均为100%,IgH-A、IgH-B、IgH-C、IgH-D、IgH-E、IgK-A、IgK-B、IgL组检出率分别为45%(18/40)、27.5%(11/40)、87.5%(35/40)、55%(22/40)、47.5%(19/40)、80%(32/40)、50%(20/40)、22.5%(9/40),IgH-C 联合 IgK-A 组合检出率为100%. 结论:BIOMED-2系统适用于石蜡包埋组织B细胞抗原受体基因重排检测,具有很高的敏感性和特异性.
目的:探討BIOMED-2繫統在檢測B細胞非霍奇金淋巴瘤中的應用價值.方法:選取石蠟包埋組織B細胞非霍奇金淋巴瘤40例、其他淋巴結增生性病變10例,提取組織DNA,應用BIOMED-2繫統中的61條引物分成9箇組彆進行PCR擴增,檢測DNA質量及抗原受體基因重排剋隆性.結果:94%(47/50)樣本DNA長度在300 bp以上,BIOMED-2繫統檢測的敏感性和特異性均為100%,IgH-A、IgH-B、IgH-C、IgH-D、IgH-E、IgK-A、IgK-B、IgL組檢齣率分彆為45%(18/40)、27.5%(11/40)、87.5%(35/40)、55%(22/40)、47.5%(19/40)、80%(32/40)、50%(20/40)、22.5%(9/40),IgH-C 聯閤 IgK-A 組閤檢齣率為100%. 結論:BIOMED-2繫統適用于石蠟包埋組織B細胞抗原受體基因重排檢測,具有很高的敏感性和特異性.
목적:탐토BIOMED-2계통재검측B세포비곽기금림파류중적응용개치.방법:선취석사포매조직B세포비곽기금림파류40례、기타림파결증생성병변10례,제취조직DNA,응용BIOMED-2계통중적61조인물분성9개조별진행PCR확증,검측DNA질량급항원수체기인중배극륭성.결과:94%(47/50)양본DNA장도재300 bp이상,BIOMED-2계통검측적민감성화특이성균위100%,IgH-A、IgH-B、IgH-C、IgH-D、IgH-E、IgK-A、IgK-B、IgL조검출솔분별위45%(18/40)、27.5%(11/40)、87.5%(35/40)、55%(22/40)、47.5%(19/40)、80%(32/40)、50%(20/40)、22.5%(9/40),IgH-C 연합 IgK-A 조합검출솔위100%. 결론:BIOMED-2계통괄용우석사포매조직B세포항원수체기인중배검측,구유흔고적민감성화특이성.
Objective: To evaluate the BIOMED-2 system in detecting the monoclonality of antigen receptor gene rearrangement in B-cell Non-Hodgkin lymphoma. Methods: Genomic DNA was extracted from paraffin-embedded specimens of 40 cases of B cell Non-Hodgkin lymphoma and 10 cases of proliferative diseases of lymph nodes, and clonality of antigen receptor gene rearrangement was assessed using the 9 groups of total 61 primers of BIOMED-2 system. Results: 94% (47/50) of specimens yielded PCR products over 300 bp using DNA quality control primers. The sensitivity and specificity of BIOMED-2 system in the detection of monoclona-lity of antigen receptor gene rearrangement were both 100%. The rate of detection of monoclonality using primes for IgH-A,IgH-B,IgH-C,IgH-D,IgH-E,IgK-A,IgK-B,IgL was 45%(18/40),27.5%(11/40),87.5%(35/40), 55%(22/40),47.5%(19/40),80%(32/40),50%(20/40) and 22.5%(9/40)respectively. When combining IgH-C and IgK-A primers, all cases of antigen receptor gene monoclonality rearrangement could be detected. Conclusion:BIOMED-2 system is a highly sensitive and specific method in detecting the monoclonality of antigen receptor gene rearrangement of B cell Non-Hodgkin lymphoma in routine paraffin embedded specimens.
