CAJ | 학술논문

目的探讨结缔组织生长因子( CTGF)对脑胶质瘤细胞增殖的影响,并分析Rho激酶信号转导通路及雷公藤单体在其中的作用. 方法取传代培养生长的脑胶质瘤细胞,按如下进行操作:正常对照组未予以任何无药物干预,CTGF组予以2.5 μg? L-1 CTGF孵育细胞24 h;雷公藤甲素组予以2.5 μg? L-1 CTGF与0.5 ng? mL-1雷公藤甲素共同孵育细胞 24 h;雷公藤红素组予以 2.5μg? L-1 CTGF与0.5 ng? mL-1雷公藤红素共同孵育细胞24 h;Y27632 ( Rho激酶特异性抑制剂)组予以1 μmol? L-1 Y27632 预处理细胞30 min后,加入2.5μg? L-1 CTGF作用24 h. 用酶联免疫吸附测定法对Rho激酶活性进行检测.用氚化胸腺嘧啶核苷法检测脑胶质瘤细胞的增殖. 结果 CTGF组脑胶质瘤细胞DNA的合成量显著高于正常对照组( P<0.01 ) ,雷公藤甲素组和雷公藤红素组脑胶质瘤细胞DNA的合成量显著低于CTGF组(P<0.05),Y27632组脑胶质瘤细胞DNA的合成量显著低于CTGF组( P<0.05 ). CTGF组的Rho激酶活性显著高于正常对照组( P<0.01 ) ,雷公藤甲素组和雷公藤红素组的Rho激酶活性显著低于CTGF组( P<0.05 ). 结论 CTGF可诱导脑胶质瘤细胞的增殖,雷公藤甲素和雷公藤红素可抑制CTGF诱导的脑胶质瘤细胞的增殖, Rho激酶信号转导通路参与CTGF诱导的脑胶质瘤细胞增殖.
목적 탐토결체조직생장인자( CTGF)대뇌효질류세포증식적영향,병분석Rho격매신호전도통로급뢰공등단체재기중적작용. 방법 취전대배양생장적뇌효질류세포,안여하진행조작:정상대조조미여이임하무약물간예,CTGF조여이2.5 μg? L-1 CTGF부육세포24 h;뢰공등갑소조여이2.5 μg? L-1 CTGF여0.5 ng? mL-1뢰공등갑소공동부육세포 24 h;뢰공등홍소조여이 2.5μg? L-1 CTGF여0.5 ng? mL-1뢰공등홍소공동부육세포24 h;Y27632 ( Rho격매특이성억제제)조여이1 μmol? L-1 Y27632 예처리세포30 min후,가입2.5μg? L-1 CTGF작용24 h. 용매련면역흡부측정법대Rho격매활성진행검측.용천화흉선밀정핵감법검측뇌효질류세포적증식. 결과 CTGF조뇌효질류세포DNA적합성량현저고우정상대조조( P<0.01 ) ,뢰공등갑소조화뢰공등홍소조뇌효질류세포DNA적합성량현저저우CTGF조(P<0.05),Y27632조뇌효질류세포DNA적합성량현저저우CTGF조( P<0.05 ). CTGF조적Rho격매활성현저고우정상대조조( P<0.01 ) ,뢰공등갑소조화뢰공등홍소조적Rho격매활성현저저우CTGF조( P<0.05 ). 결론 CTGF가유도뇌효질류세포적증식,뢰공등갑소화뢰공등홍소가억제CTGF유도적뇌효질류세포적증식, Rho격매신호전도통로삼여CTGF유도적뇌효질류세포증식.
Objective To explore the role of Rho-kinase in glioma cell proliferation induced by connective tissue growth factor ( CTGF ) and effects of triptolide/tripterine.Methods A subculture growth of glioma cells were got to take the experiment, the normal control group was not give any drug intervention .CTGF group was given CTGF 2.5 μg? L-1 and incubation for 24 h.Triptolide group was given CTGF 2.5 μg? L-1 and triptolide 0.5 ng? mL-1 and incubation for 24 h.Tripterine group were given CTGF 2.5 μg? L-1 and tripterine 0.5 ng? mL-1 and incuba-tion for 24 h.Y27632 ( group specificity of Rho kinase inhibitor ) cells after 30 min to give CTGF 2.5 μg? L -1 and Y27632 1 μmol? L-1 pre-treatment.The expressions of Rho -kinase protein were detected by ELISA.Glioma cell proliferation was measured by 3 H -TdR assay. Results CTGF could induce the proliferation of glioma cell .Triptolide, tripterine and Y27632 ( Rho kinase inhibitor ) could significantly reverse these effects ( P<0.05 ).In addition, CTGF could induce the activation of Rho-kinase ( P<0.01 ) , while triptolide and tripterine could significantly reverse these effects ( P<0.05 ) , indica-ting the activation of Rho -kinase pathway participates in glioma cell proliferation induced by CTGF . Conclusion CTGF could induce the proliferation of glioma cell , while triptolide , tripterine and Y27632 could significantly reverse these effects.Rho-kinase pathway participates in glioma cell proliferation induced by CTGF .