CAJ | 학술논문

本研究采用了巢氏 PCR 法，先以799F-1492R 引物，以大豆基因组 DNA 为模板进行扩增，将目的条带切胶回收并进行第二步PCR，其中以 GC-968F-1378R 为引物，最终得到了 V6、V7、V8三个高变区的目的片段（470bp）。采用分子生物学技术 PCR-DGGE分析大豆内生细菌多样性发现，大豆叶片中的内生细菌多样性要小于根部和茎部；大豆不同的品种间存在很多共有条带，而每个不同品种又存在各自的特有条带；在大豆的各个不同生长时期内生细菌多样性逐渐增加，只有在结荚期内生细菌多样性最小。
본연구채용료소씨 PCR 법，선이799F-1492R 인물，이대두기인조 DNA 위모판진행확증，장목적조대절효회수병진행제이보PCR，기중이 GC-968F-1378R 위인물，최종득도료 V6、V7、V8삼개고변구적목적편단（470bp）。채용분자생물학기술 PCR-DGGE분석대두내생세균다양성발현，대두협편중적내생세균다양성요소우근부화경부；대두불동적품충간존재흔다공유조대，이매개불동품충우존재각자적특유조대；재대두적각개불동생장시기내생세균다양성축점증가，지유재결협기내생세균다양성최소。
By nested PCR method and with 799F~1492R as primers, the genomic DNA of soybean has been amplified. Then we recycled the target DNA from the gel and conducted the second PCR with primers GC-968F-1378R. Finally we got the target fragment (470bp) of three high variable regions (V6, V7, V8). PCR-DGGE was used to analyze the diversity of endophytic bacteria of soybean. The diversity of endophytic bacteria in soybean leaves was less than that in roots and stems, and there were many common bands in different varieties of soybean, while each of the different varieties have their specific bands. During different growth periods of soybean the diversities of endophytic bacteria gradually increase, only in the pod bearing period endophytic bacteria diversity reached the minimum.